长期绿茶暴露改变小鼠菌状味蕾基础特征及味觉传导蛋白α-gustducin和PLCβ2的表达  

Effects of Chronic Administration of Green Tea on Anatomical Characters and the Expression of Taste Transduction Protein α-gustducin and PLCβ2 in Fungiform Taste Buds of the Mice

作　　者：熊艺姣[1] 秦玉梅[1] 郭会林[1] 段晖[1] 蔡雯雯[1] 邓少平[1] 

机构地区：[1]浙江工商大学食品与生物工程学院,杭州310035

出　　处：《中国细胞生物学学报》2013年第5期595-601,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31271820)资助的课题~~

摘　　要：以绿茶及绿茶中主要活性成分(咖啡因、茶多酚、茶氨酸)暴露成年ICR小鼠21天后,以小鼠的舌上皮为材料,运用舌上皮整体装片技术及免疫组化方法,实体显微镜和激光共聚焦显微镜下观察小鼠菌状味蕾基本解剖形态以及味觉信号传导关键蛋白α-味蛋白(α-gustducin)及磷脂酶Cβ2(phosphoinositide-specific phospholipase Cβ2,PLCβ2)的阳性细胞数量表达情况。与对照组小鼠相比,绿茶及绿茶中咖啡因+茶多酚刺激显著降低了菌状味蕾的数量;咖啡因+茶多酚与茶氨酸组显著降低了味细胞数量;而绿茶及其主要活性成分长期刺激后均显著降低了菌状味蕾的最大横截面积,同时免疫组化结果也表明,味觉信号传导关键蛋白α-味蛋白阳性细胞表达数量也显著降低;而磷脂酶Cβ2表达受绿茶和茶多酚+咖啡因影响最大。结果表明,绿茶及绿茶中各种主要活性成分的长期刺激会改变味觉感受的基础特征,而这些变化可能是长期茶叶摄入导致食物厌腻感或体重减轻的重要原因之一。In this study, adult ICR mice are used and treated with the solution of green tea and its crucial ac- tive components for 21 days, including caffeine, tea polyphenols and L-theanine. Following forementioned chronic exposure, the anatomical characters of fungiform taste buds and the expressions of taste signal transduction pro- teins, a-gustducin and phospholipase CI32 （PLCI32） in fungiform taste buds are detected by the methods of whole- mount preparation of tongue epitheliums, immunohistochemistry and laser confocal scanning microscope. The results show that the mice treated by green tea and caffeine＋tea polyphenols have less number of fungiform taste buds and PLCI32-immunopositive cells per taste bud than that of control mice. The size of fungiform taste buds and the number of ~t-gustducin-immunopositive cells per taste bud have more significant reduction in all components treated mice. The mice treated with caffeine＋tea polyphenols and L-theanine has less taste ceils per fungiform tastebud. In conclusion, chronic administration of green tea and its crucial active components changes the anatomical characters of fungiform taste buds and decreases the expression of taste signal transduction protein, which maybe response for, at least in part, the anorectic function and weight loss.

关 键 词：厌腻感 味觉 菌状味蕾 绿茶 

分 类 号：Q434[生物学—生理学]