我国不同地理群体栉江珧遗传多样性及系统发生分析  

The genetic diversity and phylogenetic studies of five populations of Atrina pectinta in China

作　　者：严加坤[1,2] 杨爱国[2] 周丽青[2] 吴彪[2] 白临建[1,2] 刘志鸿[2] 

机构地区：[1]上海海洋大学水产与生命学院,201306 [2]农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《渔业科学进展》2013年第2期29-35,共7页Progress in Fishery Sciences

基　　金：山东省科技发展计划项目(2010GHY10513);山东省自然科学基金项目(ZR2009DQ006);青岛市成果转化项目(10-3-4-17-chg);市南区2011年第二批科技发展基金项目(2011-5-023-QT)共同资助

摘　　要：利用通用引物对5个栉江珧野生群体(山东长岛、山东日照、山东文登、广东湛江和海南海口)28SrRNA和COI基因片段进行扩增测序,分别得到983bp和623bp的片段,基于28SrRNA序列分析系统发生的结果表明,栉江珧与Atrina vexillumju具有较近的遗传关系。基于COI基因序列的遗传多样性分析结果显示,山东文登群体具有最高的遗传多样性水平。AMOVA分析显示,群体遗传分化系数为0.1323(P<0.001),说明栉江珧遗传变异主要来源于群体内的变异。由28SrRNA和COI基因聚类分析结果推测,由于地理隔离,我国栉江珧南北方群体可能早已分化为不同亚种。这些数据为我国栉江珧种质资源保护和利用补充了分子生物学资料。Mitochondrial COI gene fragments and ribosomal 28S rRNA of Atrina pectinta sampled from five locations including three in Shandong （Changdao, Wendeng, and Rizhao）, one Guangdong （Zhanjiang） and one in Hainan （Haikou） were amplified with universal prim- ers, and 623bp and 983bp nucleotide sequences were obtained, respectively. Phylogenetic stud- ies of Pinnidae based on 28S rRNA partial sequences showed that A. pectinta had a close genet- ic relationship with Atrina vexillumju. Genetic diversity of the five populations based on COl partial sequences demonstrated that the Wendeng population had the highest level of genetic di- versity. The fixation indices （Fst） analyzed by AMOVA was 0. 132 3 （P〈0. 001）, indicating that the genetic variation of A. pectinta was mainly from genetic fixation inter-populations.Cluster analysis based on 28S rRNA and CO1 showed that A. pectinta in China might have dif- ferentiated into different subspecies. These findings would provide molecular biological data for the protection and utilization of A. pectinta resources in China.

关 键 词：栉江珧 28S RRNA COI 遗传多样性 系统进化 

分 类 号：S917.4[农业科学—水产科学]