合成己酸乙酯脂肪酶产生菌的产酶条件优化及其催化生产香酯液研究  

Optimization of Lipase-producing Conditions of Monascus Prupureus I518 and Research on Its Catalytic Production of Ester Liquid

作　　者：王晓丹[1,2] 胡靖[1,3] 胥思霞 吴海 张小龙[1,3] 龙茜萍[1,3] 邱树毅[1,2] 

机构地区：[1]贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]贵州大学化学与化工学院,贵州贵阳550025 [3]贵州大学生命科学学院,贵州贵阳550025 [4]贵州青酒集团有限责任公司,贵州镇远557702

出　　处：《酿酒科技》2013年第5期11-13,17,共4页Liquor-Making Science & Technology

基　　金：黔科合重大专项[2010]6004号;科技部科技支撑计划项目课题(2011BAC06B12)

摘　　要：从生产现场分离得到1株具有产己酸乙酯脂肪酶能力的红曲霉(Monascus purpureus)I518,对其产脂肪酶条件进行了初步优化。结果表明,该菌株较理想的产酶发酵条件为:麸皮∶豆粕=2∶3、接种量20%、培养基初始pH值为7、培养温度为32℃、培养时间为5 d,该条件下所产的粗酶制剂所催化的香酯液中己酸乙酯的含量达0.38 mg/mL,比未优化条件有明显增加。Monascus purpureus I518 with lipase-producing capability was obtained from a famous distillery and its lipase-producing conditions had been optimized as follows： the ratio of bran and bean pulp was 2：3, the inoculating quantity was 20 %, initial pH value in culture mediums was 7, culture temperature was at 32 ℃, and culture time was five days. The content of ethyl caproate in ester liquid catalyzed by lipase produced in the above conditions reached up to 0.38 mg/mL, evidently higher than before.

关 键 词：己酸乙酯 脂肪酶 优化 香酯液 

分 类 号：TS261.1[轻工技术与工程—发酵工程]