响应面优化金橙黄微杆菌YT9的发酵条件生产纤维素酶  

Optimization of medium conditions using response surface methodology for cellulase production by Exiguobacterium aurantiacum YT9

作　　者：程仕伟[1] 李坦坦[1] 梁会会[1] 杨立红[1] 姜国正 

机构地区：[1]鲁东大学生命科学学院应用微生物研究所,山东烟台264025 [2]烟台恒源生物股份有限公司,山东烟台265709

出　　处：《中国酿造》2013年第4期48-51,共4页China Brewing

基　　金：山东省高等学校科技计划项目(J12LD02);鲁东大学学生创新基金(12cg002)

摘　　要：利用筛选的金橙黄微杆菌发酵生产纤维素酶,对产酶影响较大的因素(培养温度、pH值和CMC添加量)进行响应面设计分析,确定最优发酵培养条件。结果表明最佳培养工艺条件为培养温度27.8℃、pH值为7.0和0.25g/L CMC添加量,纤维素酶活性达到811.75U/mL,实际值与预测值之间的误差约为0.55%,具有工业化规模生产潜力。Optimization of medium conditions using response surface methodology for cellulase production by Exiguobacterium aurantiacum YT9, which is a newly screened strain from the coastal soil samples. The results indicated that the optimal medium conditions were 27.8℃, pH value 7.0 and 0.25g/L of CMC concentration, and under these conditions the highest cellulase activity obtained was 811.75U/ml. Error of the actual value compared with the predicted was 0.55%, and Exiguobacterium aurantiacum YT9 was proved to be a suitable potential microorganism for use in cellulase production.

关 键 词：金橙黄微杆菌 纤维素酶 发酵条件 响应面分析 

分 类 号：Q815[生物学—生物工程]