正交设计法香菇菌丝体液体培养基的筛选  

Selection on liquid culture medium for mycelium of Lentinus edodes (Berk.) Sing by orthogonal design

作　　者：王红[1,2] 郭成金[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津市动植物抗性重点实验室,天津300387

出　　处：《中国酿造》2013年第4期74-77,共4页China Brewing

基　　金：国家星火计划项目(2012GA610002)

摘　　要：以香菇菌丝体生物量为主要指标,首先采用单因素试验筛选出适于香菇菌丝生长的最佳碳氮源,再进行不同比例碳氮源的正交试验,结果表明香菇菌丝液体培养的最适培养基为红糖30.00g/L、蔗糖30.00g/L、麦麸15.00g/L、牛肉膏3.00g/L、KH2PO43.00g/L、MgSO4.7H2O 1.50g/L、VB10.008g/L,pH值自然。25℃培养14d,其生物量可达4.60g/L。Adopting mycelial biomass ofLentinus edodes（Berk.） Sing as major index, firstly different carbon and nitrogen source were used in the single factor test, and then carrying out the orthogonal test for carbon source and nitrogen source ratio optimization. The result showed that the optimum liquid medium of mycelium composition of Lentinus edodes （Berk.） Sing were brown sugar 30.00g/L, sucrose 30.00g/L, wheat bran 15.00g/L, beef 3.00g/L, KH2PO4 3.00g/L, MgSO：7H2O 1.50g/L, VB1 0.008g/L, natural pH value. The biomass of mycelium ofLentinus edodes （Berk.） Sing reached 4.60g/L culturing for 14d at 25℃.

关 键 词：香菇 菌丝体 液体培养基 生物量 正交设计 

分 类 号：TS264.21[轻工技术与工程—发酵工程]