降解β-伴大豆球蛋白菌株的筛选及其发酵条件优化  

Screening of strains for degradation of soybean β-conglycinin and fermentation optimization

作　　者：朱婷[1,2] 张娟[1,2] 陈坚[3,2] 堵国成[1,2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214122 [2]江南大学生物工程学院,无锡214122 [3]江南大学粮食发酵工艺与技术国家工程实验室,无锡214122

出　　处：《食品科技》2013年第5期2-9,共8页Food Science and Technology

基　　金：国家科技支撑计划项目(2011BAK10B03);国家自然科学基金青年项目(30900013);国家自然科学基金重点项目(20836003)

摘　　要：从预制备豆制品的大豆粉浆中筛选菌株,经酪蛋白初筛及大豆分离蛋白复筛并进行SDS-PAGE验证后,得到了一株能够高效降解β-伴大豆球蛋白α亚基、α'亚基和部分β亚基的Bacillus sp.BBE201108菌株。通过单因素碳源、氮源、无机盐及正交实验确定了Bacillus sp.BBE201108的最佳培养基配方为:葡萄糖2%、大豆蛋白胨2%、酵母粉1%、Na2HPO4·12H2O0.4%、KH2PO40.06%,同时,还研究了起始pH值、培养温度对菌株产酶的影响。在最佳培养条件下,蛋白酶活力提高了3倍,最高可达292.74U/mL。A strain which can remove α-subunit,α’-subunit and partial β-subunit of β-conglycinin was isolated from soybean slurry,and then the selected strain was identified as Bacillus sp.BBE201108.The screening strategy included casein plate selecting,soybean isolate protein re-screening and SDS-PAGE verification.Based on fermentation optimization of carbon resources,nitrogen resources and orthogonal experiment,the result showed that the optimum fermentation medium consisted 2% glucose,2% soybean peptone,1% yeast extract,0.4% Na2HPO4·12H2O and 0.06% KH2PO4.Moreover,the effects of primary pH and temperature on protease production were also studied.Under the optimum conditions,protease activity was improved by 3 times,up to a maximum of 292.74 U/mL.

关 键 词：大豆过敏原 Β-伴大豆球蛋白 BACILLUS SP BBE201108 蛋白酶 发酵条件优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]