高效液相色谱法测定养殖环境中甲基睾丸酮残留量  

Determination of 17α-Methyltestosterone Residue in Water and Sediment from Tilapia Ponds by High Performance Liquid Chromatography

作　　者：邓建朝[1] 李来好[1] 杨贤庆[1] 岑剑伟[1] 吴燕燕[1] 陈胜军[1] 戚勃[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,国家水产品加工技术研发中心,广东广州510300

出　　处：《食品科学》2013年第10期187-190,共4页Food Science

基　　金：国家农业科技成果转化资金项目(2009GB2E200303;2010GB2E000335);国家现代农业产业技术体系项目(CARS-49);广东省科技计划重点项目(2008A020100006;2009B020201003);广东省海洋渔业科技推广项目(A200901C01;A201001C05)

摘　　要：建立养殖环境(水体和底泥)中甲基睾丸酮的高效液相色谱检测方法。分别以二氯甲烷提取水体中的甲基睾丸酮,甲醇提取底泥中的甲基睾丸酮,经旋转蒸发至近干,甲醇定容,紫外检测器检测,外标法定量。水体中添加质量浓度为1.00～25.0μg/L时,回收率为93.3%～101%,相对标准偏差为3.0%～5.5%,检测限为0.5μg/L。底泥中添加质量浓度为10.0～250μg/kg时,回收率为88.7%～96.6%,相对标准偏差为2.7%～6.2%,检测限为5.0μg/kg。该方法准确可行,前处理简便,适用于批量样品的测定。A high performance liquid chromatography（HPLC） method has been established for the determination of 17α-methyltestosterone（17α-MeT） in water and sediment of tilapia ponds.17α-MeT in water and sediment samples was extracted using methylene chloride and methanol,respectively,followed by rotary evaporation.Then,the residues were dissolved in methanol.Finally,17α-MeT was detected using a UV detector and quantified by external standard method.The average recovery rates of water spiked with 17α-MeT at 1.00–25.0 μg/L were 93.3%–101% with RSD of 3.0%–5.5%.The average recovery rates of sediment spiked with 17α-MeT at 10–250 μg/kg were 88.7%–96.6% with RSD of 2.7%–6.2%.The limits of detection for 17α-MeT in water and sediment were 0.5 μg/L and 5.0 μg/kg,respectively.The established method is sensitive,accurate and suitable for the determination of 17α-MeT residues in aquaculture water and sediment samples.

关 键 词：甲基睾丸酮 高效液相色谱法 养殖水体 底泥 

分 类 号：O657.72[理学—分析化学]