紫外分光光度法测定薯蓣皂甙元含量的研究  

The Study on Determination of Diosgenin Content by UV Spectrophotometry

作　　者：沈晖[1] 赵丛丛[1] 刘曾[1] 曾凡骏[1] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065

出　　处：《食品研究与开发》2013年第7期107-110,共4页Food Research and Development

摘　　要：采用紫外分光光度计测定薯蓣皂甙元含量,确定薯蓣皂甙元的最大吸收波长为452 nm,最佳实验条件为:5%香草醛-冰醋酸溶液的加量0.2 mL,高氯酸溶液的加量0.7 mL,水浴温度80℃,水浴时间15 min,放置时间20 min。精密度实验的相对标准偏差(RSD)为1.123%,加标回收率实验的平均回收率为99.5%。该方法具有操作简便、稳定性好、灵敏度高、重现性好等优点。By UV spectrophotometry the maximum wavelength of diosgenin was determined at 452 nm, and the optimized experiment conditions were studied： 5 % vanillin-glacial acetic acid solution dosage of 0.2 mL,high acid solution dosage of 0.7 mL,water bath temperature of 80 ℃ ,water bath time of 15 min, storage time of 20 min. The RSD of precision experiment was 1.123 %, and the average recovery of experiment adding standard could be 99.5 %.The method was simple, stable, sensitive, and reproduced well.

关 键 词：紫外分光法 薯蓣皂甙元 检测 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]