野龙竹竹叶中多酚含量及其抑菌活性  

A study on polyphenol content and antibacterial activities in leaves of Dendrocalamus semiscandens

作　　者：梁倩 刘蔚漪[1] 王芳[1] 赵平[1] 刘云[1] 王军民[1] 徐文晖[1] 

机构地区：[1]西南林业大学西南山地森林资源保育与利用省部共建教育部重点实验室,昆明650224

出　　处：《林业科技开发》2013年第3期112-114,共3页China Forestry Science and Technology

基　　金：国家自然科学基金(31201572);西南林业大学人才引进项目;教育部重点实验室科研基金项目(KLESWFU-1104)

摘　　要：测定了野龙竹竹叶醇提物的乙酸乙酯萃取物和水溶物两部分的多酚含量,并以枯草芽孢杆菌、四联球菌、普通变形杆菌为供试菌,采用抑菌圈法和最低抑菌浓度(MIC)法测定其抑菌活性。乙酸乙酯萃取物和水溶物两部分的多酚含量分别为8.478mg/g和0.2798mg/g;乙酸乙酯萃取物抑菌圈平均直径为17～22mm,最低抑菌浓度均为0.25mg/mL;水溶物部分的抑菌圈平均直径为6mm,最低抑菌浓度为1.0g/mL。野龙竹竹叶中含有较为丰富的多酚类物质,其乙酸乙酯萃取物具有明显的抑菌作用。The ethanol extracts from leaves of Dendrocalamus semiscandens were divided into two fractions of ethyl acetate extract and water extract. They were used to determine polyphenol content with foline - phenol. Then antibacterial activities on Proteus vulgaris ,Micrococcus tetragenus, and Bacillus subtilis of two fractions were studied by inhibition zone method and minimal inhibitory concentration （MIC） method. The polyphenol contents of ethyl acetate extract and water extract were 8. 478 mg/g, 0.279 8 mg/g respectively. The inhibition zones of ethyl acetate extract were 17-22 mm and that of water ex- tract were 6 ram. The minimal inhibitory concentration （MIC） of ethyl acetate extract for Proteus vulgaris, Micrococcus tet- ragenus, Bacillus subtilis was 0.25 mg/mL, that of water extract was 1.0 g/mL. The leaves of Dendrocalamus semiscan- dens have phenolic compounds and good bacteriostatic action.

关 键 词：野龙竹 多酚 抑菌活性 

分 类 号：TS262.5[轻工技术与工程—发酵工程]