运用PCR-DGGE技术分析原料乳中腐败细菌的多样性  

Application of PCR-DGGE in the Research of Spoilage Bacteria Diversity in Raw Milk

作　　者：唐玲[1] 王晓静 周国君 朱建荣 胡萍[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州三联乳业有限公司,贵州贵阳550025

出　　处：《贵州农业科学》2013年第5期151-154,共4页Guizhou Agricultural Sciences

基　　金：贵州省科技农业攻关项目"乳制品质量安全关键技术研究与应用示范课题"[黔科合NY字(2009)3076];贵阳市乳业重大专项"振兴贵阳牛奶产业急需关键技术研究与集成"[(2009)筑科农合同字2-017-5];贵州大学研究生创新基金"乳品腐败微生物DNA指纹图谱鉴定及污染追溯研究"(校研农2012013)

摘　　要：为揭示原料乳中主要的污染细菌种群,从原料乳中提取细菌的总DNA,PCR扩增16SrDNA的V3可变区,再通过DGGE得到指纹图谱。结果表明:原料乳中共检测出12种细菌,说明原料乳中细菌具有较丰富的种群多样性。条带经DNA测序分析表明,猪链球菌(Streptococcus suis)、马肠链球菌(Strepto-coccus equinus)和乳酸链球菌(Lactococcus lactis subsp.)是混合原料乳中的优势污染菌,其次为海豚链球菌(Streptococcus iniae)、解没食子酸链球菌(Streptococcus gallolyticus)和1株非培养的细菌(Unculturedbacterium)。To reveal the main pollution bacteria population in raw milk, DNA of total bacteria were directly extracted from the raw milk and the PCR amplification of V3 variable region of 16S rDNA were taken in order to get the fingerprint profile by DGGE technology. The results showed that 12 bacteria were detected in raw milk and indicating that there were abundant microbial population in raw milk. The DNA sequences of bands were analyzed and the dominant polluted bacteria in raw milk were found to be Streptococcus suis , Streptococcus equinus and Lactococcus lactis sub sp., Streptococcus iniae , Streptococcus gallolyticus and Uncultured bacterium secondarily.

关 键 词：PCR—DGGE技术 原料乳 腐败菌 多样性 

分 类 号：Q939.98[生物学—微生物学]