黑曲霉固态发酵生产单宁酶的条件优化  

Optimization of tannase production by solid state fermentation from Aspergillus niger

作　　者：金伟[1] 聂光军[1] 王丽[1] 王鹏[1] 贡国鸿[1] 郑之明[1] 

机构地区：[1]中国科学院离子束生物工程重点实验室,合肥230031

出　　处：《生物加工过程》2013年第3期7-12,共6页Chinese Journal of Bioprocess Engineering

基　　金：中国科学院知识创新工程项目(KSCX2-YW-G-050)

摘　　要：研究采用响应面法优化黑曲霉固态发酵生产单宁酶的培养条件。应用Plackett-Burman试验筛选出重要影响因子:五倍子粉含量、(NH4)2SO4浓度以及接种孢子量,最陡爬坡试验逼近最大响应区域。应用Box-Behnken响应面试验对重要影响因子进一步优化。得到最佳培养条件:每250 mL三角瓶中装入1.0 g五倍子粉、4.4 g稻壳和0.5 g麸皮、液固比(mL/g)2∶1且营养盐溶液组成为(NH4)2SO421 g/L、MgSO4.7H2O 1 g/L、NaCl 1 g/L,培养基pH自然,接种5.7×107个孢子后在30℃温度下培养4 d。在此条件下,单宁酶产量从40 U/g提高到114 U/g,3次重复验证性试验平均值为115 U/g,验证了模型的可靠性。Response surface methodology （RSM） was used to optimize culture conditions for tannase production by solid-state fermentation with Aspergillus niger. Plackett-Burman design （PBD） experiment screened out three significant factors：gallnut powder content, （NH4 ）2 SO4 concentration and spores number of inoculum. The steepest ascent experiment approached the greatest response space. Box- Behnken design （BBD） experiment was used to further optimize the significant factors. The optimum culture conditions for the production of tannase are as follows ： each 250 mL flask loaded 1.0 g gallnut powder,4. 4 g rice husk and 0.5 g wheat bran, liquid-solid ratio of the medium was 2： l and nutrient solution was constituted by （NH4）2SO4 21 g/L,MgSO4 ·7H2O1 g/L and NaC1 1 g/L,with a natural pH value ,the medium was inoculated with 5.7 ×10^7 spores and cultured under 30℃ for 4 days. Under those optimized culture conditions, the production of tannase increased from 40 U/g to 114 U/g. The reliability of the model was verified through triplicate experiments with an average yield of 115 U/g.

关 键 词：单宁酶 固态发酵 黑曲霉 响应面法 

分 类 号：TQ925[轻工技术与工程—发酵工程]