低能离子束辐照氨基酸结构变化的傅立叶变换红外光谱法研究  

A Study on structural changes of amino acids implanted with low energy ions by FTIR spectroscopy

作　　者：张冬梅[1] 孙素琴[1] 马志龙[1] 陈国强[1] 崔福斋[1] 

机构地区：[1]清华大学,北京100084

出　　处：《核技术》2000年第4期213-218,共6页Nuclear Techniques

基　　金：国家自然科学基金!19874034

摘　　要：利用傅立叶变换红外光谱（ＦＴＩＲ）法研究了３０ｋｅＶ的Ｎ~＋离子束辐照引起氨基酸结构的变化。结果表明：经相同剂量Ｎ~＋离子束辐照后，天冬氨酸（Ａｓｐ）和苯丙氨酸（Ｐｈｅ）的基团有相似的变化规律，即羧酸根离子（ＣＯＯ）的 Ｖ_Ｃ＝Ｏ伸缩振动（分别为１６４６ｃｍ^(－１)和１６２６ｃｍ^(－１)）均增强， ＮＨ３~＋的谱峰（分别主要为１５１７ｃｍ^(－１)和１５６０ｃｍ^(－１)）强度均减弱。 Ｐｈｅ较Ａｓｐ中的基因强度变化更明显。随注入剂量增加，Ａｓｐ的各基团相对浓度呈规律性变化。同时对产生上述变化的原因进行了探讨，并对较高剂量辐照时Ａｓｐ的分解产物进行了初步分析。The structural changes of aspartic acid (Asp) and phenylalanine (Phe) implanted with N^+ ions of 30keV were studied using Fourier transform infrared (FTLR) spectroscopy. Implanted at the same fluence of 1×10^(16)/cm^2, similar phenomena were observed in both Asp and Phe, i.e., the relative intensities of NH_3^+ vibration decreased and those of COO^- stretching vibration increased. The changes of absorption intensities in Phe were more obvious than those in Asp. It was also observed that the intensities of COOH, COO^- and NH^(3+) groups of Asp changed regularly with increasing implantation fluences. The possible reasons responsible for these results were studied. The radiolysis products of Asp implanted at a higher fiuence of 6×10^(16)/cm^2 were analyzed.

关 键 词：低能离子注入 辐照效应 FTIR 辐射 氨基酸 

分 类 号：Q517.04[生物学—生物化学] S335.21[农业科学—作物遗传育种]