中药天麻成分对灰树花胞外多糖合成及相关关键酶的影响  

Effect of Rhizoma Gastrodiae on Key Enenzyme Activities Involved in the Biosynthesis of Exopolysaccharides from Grifola frondosa in Submerged Culture

作　　者：贺宗毅[1,2] 吴天祥[2,3] 徐晓宝[3] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]贵州大学生命科学学院,贵州贵阳550025 [3]贵州大学化学与化工学院,贵州贵阳550025

出　　处：《食品科学》2013年第11期199-202,共4页Food Science

基　　金：贵州省优秀科技教育人才省长基金项目(黔省专合字(2009)106号);国家自然科学基金地区基金项目(31060272)

摘　　要：采用液体深层发酵方式研究中药天麻对灰树花胞外多糖合成的影响,并用苯酚-硫酸法测定总糖含量。结果表明:天麻添加量为7g/L时能显著促进灰树花胞外多糖产量的增加。此时灰树花产胞外多糖产量平均为3.91g/L,与对照组的3.72g/L相比提高了5.1%。同时,对灰树花多糖合成代谢途径中的两个重要酶的酶活力进行测定。说明添加天麻成分发酵后,磷酸葡萄糖异构酶酶活力有所降低,磷酸葡萄糖变位酶酶活力则无明显变化。The effect of Rhizoma Gastrodiae on the biosynthesis of expolysaccharide （EPS） from Grifolafrondosa in submerged culture was studied. Total polysaccharide content was determined by phenol-sulphuric acid method. The results showed that Rhizoma Gastrodiae at 7 g/L significantly promoted the biosynthesis of EPS in Grifolafrondosa when compared with blank control, increasing EPS yield from 3.72 g/L to 3.91 g/L. The measured activities of two key enzymes involved in the synthesis of EPS indicated that after fermentation in the presence of Rhizoma Gastrodiae, PGI activity was decreased, although a-PGM activity showed no obvious change.

关 键 词：灰树花 天麻 胞外多糖 磷酸葡萄糖异构酶 磷酸葡萄糖变位酶 

分 类 号：Q936[生物学—微生物学]