低聚果糖、乳酸杆菌对便秘模型大鼠的通便功能影响  

Aperient Effect of Fructooligosaccharides and Lactobacillus on Constipation Model of Rats

作　　者：刘晓梅[1,2] 彭芝榕[1,2] 倪学勤[1,2,3] 杨杰[1] 邹昭勇[1] 邱春红[1] 曾东[1,2,3] 杨立格[1,2] 

机构地区：[1]四川农业大学动物医学院动物微生态研究中心,四川雅安625014 [2]四川农业大学动物医学院生物工程系,四川雅安625014 [3]动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《食品科学》2013年第11期296-299,共4页Food Science

基　　金：教育部留学回国人员科研启动基金资助项目(0835-1);四川农业大学本科生科研兴趣项目(2011037)

摘　　要：通过建立Wistar大鼠便秘模型探讨低聚果糖和乳酸杆菌的通便功能。取日龄、体质量相近的Wistar雄性大鼠36只,随机选取4只作正常对照组,其余大鼠便秘造模成功后随机分成8组:模型对照组、阳性治疗组(番泻叶10.0mg/kg)及低聚果糖(1.0、0.5、0.25g/(kg·d))和乳酸杆菌(2.0×1010、2.0×109、2.0×108CFU/(kg·d))各高、中、低剂量组。连续灌喂7d,测定采食量、饮水量、体质量增量、炭末推进率、粪便粒数及肠道菌群6项指标。低聚果糖和乳酸杆菌均具有润肠通便的功用,大鼠的采食量、饮水量、体质量增量、炭末推进率和粪便粒数5项指标均显著高于模型对照组(P<0.05);同时,都能抑制大鼠肠道内肠球菌与肠杆菌的生长繁殖(P<0.05);最佳剂量分别为1.0g/(kg·d)和2.0×108CFU/(kg·d)。低聚果糖和乳酸杆菌均具有润肠通便作用且对维持肠道菌群平衡、抑制肠球菌和肠杆菌的生长有较好功效。To explore the defecating function of fructooligosaccharide （FOS） and Lactobacillus, constipation model of Wistar rats was established. From 36 male wistar rats of the same age and weight, 4 rats were randomly selected as blank control group, while the remaining rats were used as constipation model and randomly divided into 8 groups, including model group, positive treatment group （folium senna 10.0 mg/kg）, FOS high, medium and low-dose groups （corresponding to 1.0, 0.5, 0.25 g/（kg·d） and Lactobacillus high, medium and low-dose groups（corresponding to 2.0 × 10^10, 2.0 × 10^9, 2.0 ×10^8 CFU/（kg·d）. After continuous force-feeding for 7 days, 6 indexes, i.e., feed intake, water intake, body mass increment, the advance rate of carbon powder, fecal pellets and intestinal flora, were detected. Both FOS and Lactobacillus had defecating function. Water intake, body mass increment, the advance rate of carbon powder and fecal pellets of the 6 groups with garage of FOS or Lactobacillus were significantly higher than those of model group （P 〈 0.05）. Besides, FOS and Lactobacillus had an inhibiting effect on the growth of intestinal Enterococci and Enterobacteriaceae （P 〈 0.05）. The optimal dosage of FOS and Lactobacillus was 1.0 g/（kg·d） and 2.0 × 10^8 CFU/（kg·d）, respectively. Both FOS and Lactobacillus have defecating function, maintain the balance of intestinal flora and inhibit the growth of intestinal Enterococci and Enterobacteriaceae.

关 键 词：低聚果糖 乳酸杆菌 便秘 通便作用 WISTAR大鼠 

分 类 号：R574[医药卫生—消化系统]