酶解猪皮明胶生产抗氧化肽工艺的研究  

Research of Producing Process on Enzymatic Preparation Anti-oxidation Peptides from Pigskin Gelatin

作　　者：张小强[1,2] 董文宾[2] 

机构地区：[1]咸阳师范学院体育系咸阳712000 [2]陕西科技大学生命科学与工程学院西安710021

出　　处：《食品工业》2013年第6期47-49,共3页The Food Industry

基　　金：明胶对疲劳动物CNT和线粒体电子漏代谢的调节作用和分子转导机制(编号:SUST-KJCH4880);咸阳师范学院专项科研基金重点项目,编号12XSYK083

摘　　要：通过对六种酶水解明胶产物清除O2-.功效的比较,筛选中性蛋白酶为水解用酶。进一步在单因素试验的基础上,通过响应面试验优化得到最佳酶解条件为温度43.65℃,pH 6.25,时间3.29 h,酶质量分数3%。在此条件下水解明胶,对酶解产物进行体外超氧阴离子清除活性分析,理论清除率为72.01%,试验值为71.15%。The different protease used to hydrolyze 02- radical scavenging rate is a filtering factor. Next, neutral protease was used to hydrolyze pigskin gelatin. The hydrolysis conditions were optimized by response surface methodology on the basis of single factor experiments. The optimized conditions were as follows; hydrolysis temperature 43.65 ℃, pH 6.25, time 3.29 h, alcalase content 3%. Under the conditions, the O2- scavenging rate of the hydrolysis was up to 72.01%. The experi- mental results are 71.15%.

关 键 词：猪皮明胶 中性蛋白酶 响应面分析 超氧阴离子自由基 

分 类 号：TS201.2[轻工技术与工程—食品科学]