Nanoparticles carrying neurotrophin-3-modified Schwann cells promote repair of sciatic nerve defects  

作　　者：Haibin Zong Hongxing Zhao Yilei Zhao Jingling Jia Libin Yang Chao Ma Yang Zhang Yuzhen Dong 

机构地区：[1]Functional Laboratory,School of Basic Medical Sciences,Xinxiang Medical College [2]Department of Orthopedics,the First Affiliated Hospital of Xinxiang Medical College

出　　处：《Neural Regeneration Research》2013年第14期1262-1268,共7页中国神经再生研究（英文版）

基　　金：financially supported by the grants for Science and Technology Innovation Talents in Universities in Ministry of Education of Henan Province in 2010,No.2010HASTIT036

摘　　要：Schwann ceils and neurotrophin-3 play an important role in neural regeneration, but the secretion of neurotrophin-3 from Schwann cells is limited, and exogenous neurotrophin-3 is inactived easily in vivo. In this study, we have transfected neurotrophin-3 into Schwann cells cultured in vitro using nanoparticle liposomes. Results showed that neurotrophin-3 was successfully transfected into Schwann cells, where it was expressed effectively and steadily. A composite of Schwann cells transfected with neurotrophin-3 and poly（lactic-co-glycolic acid） biodegradable conduits was transplanted into rats to repair 10-mm sciatic nerve defects. Transplantation of the composite scaffold could restore the myoelectricity and wave amplitude of the sciatic nerve by electrophysiological examination, promote nerve axonal and myelin regeneration, and delay apoptosis of spinal motor neurons. Experimental findings indicate that neurotrophin-3 transfected Schwann cells combined with bridge grafting can promote neural regeneration and functional recovery after nerve injury.Schwann ceils and neurotrophin-3 play an important role in neural regeneration, but the secretion of neurotrophin-3 from Schwann cells is limited, and exogenous neurotrophin-3 is inactived easily in vivo. In this study, we have transfected neurotrophin-3 into Schwann cells cultured in vitro using nanoparticle liposomes. Results showed that neurotrophin-3 was successfully transfected into Schwann cells, where it was expressed effectively and steadily. A composite of Schwann cells transfected with neurotrophin-3 and poly（lactic-co-glycolic acid） biodegradable conduits was transplanted into rats to repair 10-mm sciatic nerve defects. Transplantation of the composite scaffold could restore the myoelectricity and wave amplitude of the sciatic nerve by electrophysiological examination, promote nerve axonal and myelin regeneration, and delay apoptosis of spinal motor neurons. Experimental findings indicate that neurotrophin-3 transfected Schwann cells combined with bridge grafting can promote neural regeneration and functional recovery after nerve injury.

关 键 词：neural regeneration peripheral nerve injury NEUROTROPHIN-3 nanoparticle liposome Schwanncells sciatic nerve neuroelectrophysiology gene transfection grants-supported paper NEUROREGENERATION 

分 类 号：R745[医药卫生—神经病学与精神病学]