乌骨鸡黑色素氧化降解产物成分初探  

Analysis of oxidative degradation product of Melanin from Black-bone Silky Fowl

作　　者：田颖刚[1] 赵溪雁[1] 乔娟娟[1] 朱胜[1] 张丽[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品工业科技》2013年第12期86-89,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(20962015);江西省自然科学基金项目(2009GZN0087);江西省教育厅科技项目(GJJ10308)

摘　　要：采用蛋白酶/盐酸法分离获得乌骨鸡黑色素,并对其进行透射电镜观察,以NaOH-H2O2氧化法对其进行降解,用乙醚萃取降解产物,对萃取物进行傅里叶红外光谱、GC/MS分析。结果显示,蛋白酶/盐酸法提取的乌骨鸡黑色素呈游离的椭球形、无明显附着物;傅里叶红外光谱在2960～2850cm-1、1470～1370cm-1范围内有强吸收峰,提示降解产物中可能存有烷基基团;通过GC/MS分析发现,乌骨鸡黑色素乙醚萃取物中不含烷烃,乌骨鸡黑色素降解产物中存在C16～C32一系列不同碳链长度烷烃,且烷烃含量随着降解时间的延长而增加,这表明烷烃是在降解过程中逐渐产生的,推测乌骨鸡黑色素结构中可能存有不同链长的脂肪族结构。Melanin from Black-bone Silky FowI（BSFM） was extracted with proteolytic hydrolysis and hydrochloric acid refining. And it was observed by Transmission Electron Microscope. The melanin was oxidatively degraded with NaOH-H2O2,then Fourier Transform Infrared Spectroscopy and GC/MS were used for analyzing ethereal extracts of degradation products. The results showed that the melanin was free spheroidal without any impurity in the surrounding. Infrared spectroscopy of the ethereal extract of degradation products had strong absorption peaks in the range of 2950 N2850cm -1,1470 ~1370cm -1, indicating alkyl group in ethereal extract. Meanwhile,a series of carbon alkanes（C16-C32） with different length were found in the ethereal extracts from products of different degradation time by GC/MS,and the content of alkanes in the ethereal extracts increased with the degradation time. These showed that carbon alkanes were gradually produced in the degradation process,suggesting that the aliphatics may be the part of BSFM structure.

关 键 词：乌骨鸡黑色素 氧化降解 气质联用 烷烃 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]