野生柳叶蜡梅叶挥发油成分的GC-MS分析及其抗氧化活性  

Study on chemical constituents of volatile oil from leaf of wild Chimonanthus salicifolicus with GC-MS analysis and their antioxidant activity

作　　者：周婧[1] 钱超[1] 宋莹莹[1] 张艳敏[1] 毕淑峰[1] 

机构地区：[1]黄山学院生命与环境学院,安徽黄山245041

出　　处：《华西药学杂志》2013年第3期238-240,共3页West China Journal of Pharmaceutical Sciences

基　　金：中国博士后基金面上项目(2011M500898);黄山学院博士启动基金项目(2012xkjq002);黄山学院大学生创新基金项目(2012xdkj017)

摘　　要：目的研究野生柳叶蜡梅挥发油的化学成分及其抗氧化活性。方法利用GC-MS分析挥发油的化学成分,测定各成分的相对含量;以分光光度法测定2%挥发油对ABTS自由基和亚硝酸钠的清除能力。结果从挥发油中共鉴定了62个化合物,占挥发油总量的83.13%。2%挥发油对ABTS自由基有一定的清除作用,但低于0.5 mg.mL-1的Vit C;对亚硝酸钠的清除作用高于0.5 mg.mL-1的芦丁。结论柳叶蜡梅挥发油以单萜、倍半萜为主,具有较好的抗氧化活性。OBJECTIVE To study chemical constituents of volatile oil from leaf of wild Chimonanthus salicifolicus and their antioxidant activity.METHODS The volatile oil from Chimonanthus saslicifolicus was analyzed by GC-MS and the relative contents of these components were determined with peak area normalization method.The scavenging capacity against ABTS free radical and sodium nitrite of 2% volatile oil was measured with spectrophotometer.RESULTS Sixty-two compounds,which occupied 83.13% of total constituents,were identified.The scavenging capacity against ABTS free radical of 2% volatile oil was lower than that of 0.5 mg·mL-1 Vit C,while the scavenging capacity against sodium nitrite of 2% volatile oil was higher than that of 0.5 mg·mL-1 Rutin.CONCLUSION The volatile oil from leaf of wild Chimonanthus salicifolicus was mainly composed of monoterpene and sesquiterpene and has antioxidant activity.

关 键 词：柳叶蜡梅 挥发油 气质联用 抗氧化活性 

分 类 号：R914[医药卫生—药物化学]