鸭疫里氏杆菌吉林分离株16S rRNA系统分析及其OmpA基因克隆  

Systematic Analysis of 16S rRNA and Cloning of OmpA Gene of the Riemerella anatipestifer Isolated from Jilin

作　　者：高云航[1] 刘佳丽[1] 王巍[1] 徐凤宇[1] 张福君 马红霞[1] 

机构地区：[1]吉林农业大学动物科技学院,长春130118 [2]吉林正方农牧股份有限公司,吉林梅河135000

出　　处：《中国兽药杂志》2013年第6期9-13,共5页Chinese Journal of Veterinary Drug

基　　金：吉林省科技发展计划项目(20110221);国家现代农业产业技术体系建设专项(CARS-43)

摘　　要：为研究鸭疫里氏杆菌(RA)16S rRNA变异与OmpA基因之间的关系,提取了RA吉林分离株JL-RA1和JL-RA3总DNA,并设计特异性引物扩增两株RA保护性抗原OmpA全基因序列。结果显示,扩增出的16S rRNA序列大小均为1478 bp,OmpA片段序列大小均为1164 bp,与预期结果一致。扩增的16S rRNA与GenBank中已知的RA 16S rRNA序列同源性高达99.0%～99.9%,扩增的OmpA序列与GenBank中已知的RA OmpA序列同源性达93.3%～100%,编码蛋白质的氨基酸序列同源性为96%～100%。For future study of the relationship between the variation of 16S rRNA with the OmpA gene of Riemerella anatipestifer（ RA）, the total DNA of the RA that were isolated from Jilin which named JL - RA1 and JL-RA3 was extracted, the 16S rRNA sequence was amplified by bacterial universal primers, and both of the RA protective antigen OmpA gene were amplified by the specific primers. Results showed that the 16S rRNA sequences were 1478 bp, and the OmpA sequences were 1164 bp. The homology of the RA 16S rRNA sequences with those in GenBank was from 99.0% to 99.9% ,the homology of OmpA sequences was from 93.3% to 100% , and the homology of amino acid sequence that encoding proteins was from 96% to 100%.

关 键 词：鸭疫里氏杆菌 16SRRNA基因 OmpA基因 系统进化分析 

分 类 号：S852.61[农业科学—基础兽医学]