光度法测定小麦粉中过氧化苯甲酰研究  

Spectrophotometric Determination of Flour Benzoyl Peroxide

作　　者：田林双[1] 于伟[2] 吴存兵[1] 顾鹏程[1] 周琳琨[1] 邱琳[1] 

机构地区：[1]江苏财经职业技术学院,淮安223003 [2]江苏省淮安市科技局,淮安223001

出　　处：《中国粮油学报》2013年第6期96-100,110,共6页Journal of the Chinese Cereals and Oils Association

基　　金：淮安市科技支撑计划项目(农业)项目(SN12046);江苏省高等学校大学生实践创新训练计划项目(2012JSSPITP4250);淮安市科技支撑计划项目(工业)指导项目(HAGZ2011001)

摘　　要：基于过氧化苯甲酰的氧化性,将Fe2+氧化成Fe3+,生成的Fe3+与硫氰酸盐在酸性乙醇介质中发生显色反应,建立小麦粉违禁添加剂过氧化苯甲酰检测新方法。对该方法进行试验条件研究,包括光谱吸收曲线、反应介质、酸含量、反应温度、反应时间、Fe2+浓度、KSCN浓度、共存干扰物质等因素对检测结果的影响。最佳试验条件下,过氧化苯甲酰线性范围0.1～15 mg/L,相关系数R2=0.999 4,505 nm处表观摩尔吸光系数ε=2.136 5×104L.mol-1.cm-1,检出限0.025 mg/L。利用该方法对市售小麦粉样品进行测定,回收率在97.2%～104.4%之间。A new method for benzoyl peroxide detection was proposed. It was based on the principle that benzoyl peroxide oxidize ferrous iron to ferric ion, then color reaction between ferric ion and thiocyanate in ethanol and acid system can be quantified. Experimental conditions of the benzoyl peroxide detection method including absorption curve, reaction medium, acid content, reaction temperature, reaction time, ferrous iron content, thiocyanate contentand interfering substances were studied in the research. Under the optimum conditions, the linear range of benzoyl peroxide concentration was 0.1 - 15 mg/L, with a correlation coefficient （ R2 ） of 0.999 4 and a lower detection limit of 0. 025 mg/L. The apparent molar adsorptivity of the coordination complex was 2.136 5×10^4L·mol-1·cm^-1 The recovery was at the range of 97.2% to 104.4% by this method to detect commercial flour samples.

关 键 词：过氧化苯甲酰 小麦粉 检测 

分 类 号：TS211.7[轻工技术与工程—粮食、油脂及植物蛋白工程]