裂殖壶菌单细胞油脂的组分特性分析  

Component characteristics of single cell lipid produced by Schizochytrium sp.

作　　者：孙冠男[1] 任路静[1] 瞿亮[1] 李娟[1] 胡元维[1] 黄和[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009

出　　处：《中国油脂》2013年第6期70-73,共4页China Oils and Fats

基　　金：国家科技支撑计划(2011BAD23B03);国家"863"项目(SS2012AA021704);国家"973"计划项目(2009CB724700);江苏省自然科学基金(BK2012424)

摘　　要：为了获得完整的裂殖壶菌(Schizochytrium sp.)油脂组分信息,以其单细胞油脂组分为研究对象,对裂殖壶菌单细胞油脂进行深入研究。结果表明:裂殖壶菌单细胞油脂占细胞干重的63.32%;单细胞油脂由87.864%的中性脂和11.322%的极性脂组成;单细胞油脂中DHA含量为41.45%;单细胞油脂中胆固醇、角鲨烯和β-胡萝卜素含量分别为40.93、19.99 mg/g和34.91μg/g。经气相色谱-质谱联用分析表明:单细胞油脂的不皂化物中含有胆固醇、麦角固醇、豆甾醇、β-谷甾醇、环阿屯醇和角鲨烯。In order to obtain the complete lipid component characteristics of Schizochytrium sp. , the single cell lipid component was investigated. The results showed that the content of single cell lipid in dry cell was up to 63.32% ; the single cell lipid contained 11. 322% of polar lipid and 87. 864% of neutral lipid ; DHA content in the single cell lipid was 41.45% ; the contents of cholesterol, squalene and β- carotene in the single cell lipid were 40.93, 19.99 mg/g and 34.91 p,g/g, respectively. GC - MS analysis re- vealed that the unsaponifiable matters of the single cell lipid contained cholesterol, ergosterol, stigmaster- ol, β- sitosterol, cycloartenol and squalene.

关 键 词：DHA 裂殖壶菌 单细胞油脂 不皂化物 胆固醇 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]