核桃青皮次生物质的抑菌活性研究  

Bacteriostasic activity of walnut green husk secondary metabolites

作　　者：丁存宝[1] 李桂秋 刘海燕[3] 贾长虹[1] 常丽新[1] 

机构地区：[1]河北联合大学生命科学学院,唐山063009 [2]遵化市西郊医院,遵化064200 [3]河北联合大学化学工程学院,唐山063009

出　　处：《食品科技》2013年第6期223-226,共4页Food Science and Technology

基　　金：河北联合大学博士启动基金项目

摘　　要：核桃青皮中含有抑菌活性的次生物质。通过测定核桃青皮提取物对各类病原菌的抑菌活性及最小抑制浓度,全面评价核桃青皮的抑菌活性。以核桃青皮为原料,分别用95%乙醇、石油醚、乙酸乙酯和正丁醇浸提,得到含核桃青皮的次生物质的提取液。选取大肠杆菌、白色葡萄球菌和伤寒杆菌等重要指示菌为标靶,观察不同溶剂萃取物对指示菌的抑制作用,从而研究核桃青皮的次生物质的体外抑菌活性。利用分光光度法对核桃青皮提取液中的多糖、胡桃醌和黄酮含量进行测定,计算出提取液中各成分的浓度,从而得出对细菌抑制的主要成分。结果显示:核桃青皮各种提取液对各指示菌的抑制效果为95%乙醇>乙酸乙酯>正丁醇>石油醚。95%乙醇提取液的抑菌活性最为显著,石油醚提取液对核桃青皮提取液的抑菌活性最弱。其中核桃青皮的次生物质中胡桃醌在抑菌作用中起主要作用。Determined the minimum inhibitory concentrations for bacteria of walnut green husk extracts. Walnut green husk as materials, secondary metabolites of walnut green husk was extracted with ethanol, petroleum ether, ethyl acetate and n-butyl alcohol. Using Escherichia coli, Staphylococcus albus, Salmonella Typhi as target and important directives the bacteriostasic activity of different solvent extracts was studied. Using the spectrophotometer, polysaccharides, flavone and juglone were detected in walnut green husk extraction. Results： The bacteriostasic activity of different solvent extracts： 95% ethyl alcohol〉ethyl acetate〉n-butyl alcohol〉petroleum ether. The bacteriostasis activity of 95% ethyl alcohol extracts is most remarkable, petroleum ether extracts is weakest. Juglone was the effective ingredients in secondary metabolites of walnut green husk in bacteriostasis activity.

关 键 词：核桃青皮 抑菌活性 提取 

分 类 号：TS202.3[轻工技术与工程—食品科学]