密蒙花黄色素的提取及其紫外可见光谱指纹图谱研究  

Extraction and the ultraviolet visible fingerprinting spectrum of yellow pigment from Buddleja officinalis

作　　者：杨胜远[1] 李卓文[1] 方晓旋[1] 赖演莲[1] 陈晓屏[1] 杨娜[1] 

机构地区：[1]韩山师范学院生物系食品与发酵工程研究所,潮州521041

出　　处：《食品科技》2013年第6期281-285,共5页Food Science and Technology

基　　金：国家星火计划项目(2011GA780022);潮州市科技引导计划项目(2011N01);韩山师范学院教授科研启动项目(QD20110304)

摘　　要：对密蒙花花序的花、苞片和花轴黄色素的色价进行了分析,结果表明密蒙花黄色素主要存在于花,色价为(2860.90±41.67)U/g。经单因素试验和正交试验优化,黄色素的适宜提取条件为:乙醇浓度70%、浸提温度60℃、料液比1:20、浸提时间15min,提取率为(96.67±1.44)%,提取液冻干粉的色价为(3750±280)U/g,比商品柠檬黄色素的色价低7倍。密蒙花黄色素在pH3～9.18范围内随着pH值升高,328nm和435nm处的2个峰逐渐向371nm处移动汇集形成新的特征峰,预期这一特性可作为密蒙花的指纹图谱用于对密蒙花植物或密蒙花黄色素的鉴别。The color value of yellow pigments in the flowers, bracts and floral axes of butterflybush flower inflorescence was analyzed. The results indicated the yellow pigments mostly exist in the flowers, which color value was （2860.90±41.67） U/g. The results of single factor test and orthogonal test show that the optimal conditions for the yellow pigments extraction from butterflybush flower were： ethanol 70%, temperature 60 %, the ratio of butterflybush flower power to solution 1：20, and time 15 min. Under the optimal conditions, （96.67±1.44）% of yellow pigments were extracted, and the color value of the lyophilized powder from the butterflybush flowers extraction was （3750±280） U/g, which was 7-folds less than the commercial lemon yellow pigments. At the rang of pH3 to pH9.18, the characteristic peaks of butterflybush flowers yellow pigments at 328 nm and 435 nm would gradually move closer with the increase of pH value, and finally integrate to a new peak at 371 nm. The change regulation of ultraviolet visible spectrum of butterflybush flower yellow pigments would be expected to be used as the fingerprinting spectrum for identification of the plant or yellow pigments of butterflybush flower.

关 键 词：密蒙花 黄色素 提取 指纹图谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]