液态发酵细脚拟青霉培养基及其发酵条件的优化  

The optimization of liquid culture medium and conditions for paecilomyces tenuipes

作　　者：陈剑翔[1] 张庆庆[1] 金鑫强[1] 赵爱斌[1] 李慧静[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000

出　　处：《安徽工程大学学报》2013年第2期7-10,共4页Journal of Anhui Polytechnic University

摘　　要：对细脚拟青霉进行液态培养,采用单因素实验和正交实验对培养基及培养条件进行优化.结果表明,培养基组成为:4%葡萄糖,2%蚕蛹粉,0.15%KH2PO4,0.15%MgSO4.7H2O;培养条件为:接种量5%,温度25℃,初始pH值6.5,摇床转速140r/min,250mL三角烧瓶中装液量为80mL.细脚拟青霉的菌丝生物量优化后产量提高了18.9%.mized by single factor and orthogonal experimental design. The results showed that cultivation medium composition was as follows;4% glucose, 2% Silkworm chrysalis powder, 0. 15% KHzPO4, 0.15% MgSO4 · 7 H2 O. Cu）.ture condition was.the inoculum size 5 %, temperature 25 ℃, the initial pH 6.5, rotation speed 140 r/min,250 mL flask installed with liquid volume of 80 mL. The biomass of Paecilomyces tenuipes mycelial increased by 18.9%.

关 键 词：细脚拟青霉 蚕蛹 液态培养 正交实验 

分 类 号：TS201.3[轻工技术与工程—食品科学]