大孔树脂DA201-C对猪血红蛋白ACE抑制肽吸附性能的研究  

Macroporous Resin Adsorption for Separation and Purification of ACE Inhibitory Peptides from Porcine Hemoglobin

作　　者：邓惠玲[1] 郑炯[1,2] 阚建全[1,2] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]重庆市农产品加工及贮藏重点实验室,重庆400715

出　　处：《食品科学》2013年第12期27-31,共5页Food Science

基　　金：中央高校基本科研业务费专项(XDJK2009C055)

摘　　要：以猪血红蛋白的胃蛋白酶水解液为原料,通过静态和动态实验,对适合分离纯化猪血红蛋白ACE抑制肽的大孔树脂进行筛选,并确定最佳的分离纯化工艺参数。结果表明:DA201-C分离纯化猪血红蛋白ACE抑制肽的效果最好;动态实验最佳工艺参数为上样质量浓度40mg/mL、pH5.0、上样流速0.5mL/min、上样量60mL;洗脱液乙醇体积分数75%,洗脱流速1.0mL/min,在此条件下,分离得到的猪血红蛋白ACE抑制肽样品的IC50为0.87mg/mL。同时,对DA201-C大孔树脂进行静态吸附-解吸实验和动态吸附-解吸实验的进行了比较,结果表明:大孔树脂静态吸附率为59.4%,大于其动态吸附量48.1%。Based on static and dynamic adsorption performance, DA201-C type macroporous resin was selected as a more appropriate carrier to separate and purify ACE inhibitory peptides from the pepsin hydrolysate of porcine hemoglobin. The optimal process parameters for dynamic adsorption and desorption were determined as follows： 60 mL of 40 mg/mL samples at pH 5.0 and 0.5 mL/min of loading flow rate, followed by desorption with 75% ethanol at a flow rate of 1.0 mL/min. The IC50 of the ACE inhibitory peptides obtained under the optimized conditions was 0.87 mg/mL. Comparison of static adsorption and desorption with dynamic adsorption and desorption indicated that the static adsorption rate of ACE inhibitory peptides on DA201-C was 59.4%, which was higher than the dynamci adsorption rate of 48.1%.

关 键 词：大孔树脂 吸附 猪血红蛋白 DA201-C ACE抑制肽 

分 类 号：S879.7[农业科学—畜牧兽医]