地衣芽孢杆菌产β-甘露聚糖酶分批发酵动力学模型的建立  

Construction of Kinetics Model for β-mannase Fermentation Process Using Bacillus Licheniformis HDYM-04

作　　者：田雪[1] 解鑫[1] 周晓杭[1] 葛菁萍[1] 凌宏志[1] 宋刚[1] 平文祥[1] 

机构地区：[1]微生物黑龙江省高校重点试验室/黑龙江大学生命科学学院,哈尔滨150080

出　　处：《中国农学通报》2013年第18期193-199,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制"(31270534);国家自然科学基金"拮抗菌株对副干酪乳杆菌产细菌素的影响及关键蛋白质的筛选"(31210103026);黑龙江大学高层次人才(创新团队)支持计划"功能微生物选育及其产物应用"(Hdtd2010-17)

摘　　要：为提高β-甘露聚糖酶产量,探究其发酵生产的规律,以β-甘露聚糖酶高产菌株地衣芽孢杆菌(Bacillus licheniformis)HDYM-04为供试菌株,以初始魔芋粉加入量2%、接种量6.7%、初始pH8.0、培养温度37℃、搅拌速率300r/min、通气量3L/min、发酵周期30h为基础发酵条件进行5L发酵罐发酵。基于Logistic方程和Luedeking-Piret等方程建立了该菌株的菌体生长、产物生成、底物消耗3个分批发酵动力学模型,其中菌体生长动力学模型为:dX/dt=0.431X(1-X/15.522)、底物消耗动力学模型为-dS/dt=1.11dX/dt+0.0002dP/dt+0.0008X、产物生成动力学模型为dP/dt=133.1dX/dt+222.87X,其相关系数R2分别为0.99021、0.98908、0.98812,能真实描述菌体发酵过程中菌体生长、酶的合成以及底物消耗的情况。Abstract： In order to increase the production of β-mannase and research its regular pattern during fermentation process, high β-mannase producing strain, Bacillus licheniiormis HDYM-04, was used to investigate the kinetics model based on the optimal fermentation conditions： the strain was fermentated at 37℃for 30 h with agitation speed at 300 r/min and aeration rate at 3 L/rain in a 5 L fermantor, the initial addition of konjac flour was 2% （w/v） and the initial pH value of medium was 8.0, the inoculum was 6.7% （v/v）. The batch fermentation kinetic models were established （cell growth kinetic model, substrate depletion kinetic model, production formation kinetic model） bases on Logistic and Luedeking-Piret equations. Cell growth kinetic model was dx/dt=0.431X（1-X/15.522）subatrate depletion kinetic model was -dS/dt=1.1ldx/dt＋0.0002dp/dt＋0.0008X, and production formation kinetic model was dP/dt=133.1dx/dt＋222.87x. The correlation coefficients R2 of the three equations were 0.99021, 0.98908 and 0.98812, respectively, which indicated a good correlation between experimental values and models. Therefore, the equations could be used to describe the process of cell growth, enzyme synthesis and substrate consumption by HDYM-04 during batch fermentation. The establishment of batch fermentation kinetic models （cell growthkinetic model, substrate depletion kinetic model, production formation kinetic model） could lay a foundation for theory and provide practical nstruction for the application of HDYM-04 in industry.

关 键 词：地衣芽孢杆菌 Β-甘露聚糖酶 发酵动力学 分批发酵 

分 类 号：Q93[生物学—微生物学]