伞枝犁头霉发酵蔗糖产α-酮戊二酸条件的优化  

Optimization of fermentation conditions of α-ketoglutarate production from sucrose by Absidia corymbifera

作　　者：闫巧娟[1] 徐忠义[1] 蔡威[1] 杨绍青[2] 张建志[1] 江正强[2] 

机构地区：[1]中国农业大学工学院,北京100083 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国农业大学学报》2013年第4期162-167,共6页Journal of China Agricultural University

基　　金：中央高校基本科研业务费专项资金资助(2012QJ012)

摘　　要：研究伞枝犁头霉D1利用蔗糖生产α-酮戊二酸的发酵条件优化。结果表明:1)最佳发酵培养基组成为:蔗糖40g/L,NaNO31g/L,KH2PO41.36g/L,MgSO4.7H2O 0.2g/L,FeSO4.7H2O 0.01g/L,NaCl 2g/L,玉米浆0.5g/L,CaCO340g/L,叶酸2g/L。2)最佳培养条件为:250mL摇瓶装液25%,培养温度37℃和转速200r/min。在此条件下培养84h,伞枝犁头霉产α-酮戊二酸量最大达14.0g/L,产酸速率为0.17g/(L.h),对蔗糖的转化率为34.9%。伞枝犁头霉D1能够有效利用蔗糖发酵产α-酮戊二酸,具有较好的科研价值和工业应用前景。The present work studied the optimization of α-ketoglutarate production from sucrose by Absidia corymbifera（Lichtheimia corymbifera） D1.Based on single factor experiments,the optimum fermentation medium was found to be 40 g/L sucrose,1 g/L NaNO3,1.36 g/L KH2PO4,0.2 g/L MgSO4·7H2O,0.01 g/L FeSO4·7H2O,2 g/L NaCl,0.5 g/L corn syrup and 40 g/L CaCO3;and the optimal incubation conditions were：25% loading volume of 250 mL flask at 37℃,200 r/min for 84 h.Under the optimal fermentation conditions,the production of α-ketoglutaric acid from sucrose was up to 14.0 g/L,with a conversion of 34.9%.The volumetric production rate was up to 0.17 g/（L·h）.Hence,Absidia corymbifera D1 was efficient in utilizing sucrose for α-ketoglutarate production,which shows scientific value and a good prospect for industrial applications.

关 键 词：伞枝犁头霉 a一酮戊二酸 蔗糖 发酵条件 

分 类 号：TQ921[轻工技术与工程—发酵工程]