超高效液相色谱法测定乳及乳制品中多种磺胺类药物残留  

Determination of various Sulfonamide Residues in milk and dairy products by Ultra Performance Liquid Chromatography

作　　者：亢美娟[1,2] 宋戈[2] 王剑飞[2] 

机构地区：[1]东北农业大学食品学院,哈尔滨150028 [2]东北农业大学黑龙江省乳品工业技术开发中心,哈尔滨150028

出　　处：《中国乳品工业》2013年第6期46-48,共3页China Dairy Industry

摘　　要：建立了超高效液相色谱法同时测定乳及乳制品中14种磺胺类药物残留的方法。样品通过乙腈重复提取,饱和正己烷脱脂后浓缩,基质固相分散萃取技术净化,采用ACQUITY UPLCTM BEH C18柱,以甲醇和质量分数0.1%甲酸水溶液为流动相梯度洗脱,用紫外检测器于270 nm波长处检测。回收率在90.4%～104.3%,精密度(RSD)为1.09%～5.73%,检出限分别为0.04～0.09μg/g。该方法可用于乳及乳制品中14种磺胺类药物残留的同时测定。A method for Simultaneous determination of 14 sulfonamide residues in milk and dairy products by ultra performance liquid chromatography （UPLC） is presented. Extracted with acetonitrile, dried under nitrogen stream, defatted with n-hexane saturated by acetonitrile, enriched and purified, separated on an ACQUITY UPLCTM BEH C18 column with a mixed solution of methanol-0.1% formic acid as mo- bile phase by gradient elution, and detected at 270 rim with a ultraviolet detector. The recovery were 90.4%~104.3%,the relative standard deviations （RSD） were 1.09%~5.73%, the minimum detectable values were 0.04-0.09 μg/g. This method can be used for the routine analysis of these residues in milk and dairy products.

关 键 词：磺胺类药物残留 乳及乳制品 超高效液相色谱 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]