固定化木瓜蛋白酶水解大豆乳清废水研究  

Study on immobilized papain enzymetic hydrolyzing soybean isolated protein whey wastewater

作　　者：杨頔 张多英[2] 石彦国[3] 

机构地区：[1]黑龙江省轻工设计院,哈尔滨150040 [2]食品与环境工程学部,黑龙江东方学院,哈尔滨150086 [3]食品工程学院,哈尔滨商业大学,哈尔滨150076

出　　处：《东北农业大学学报》2013年第5期14-17,共4页Journal of Northeast Agricultural University

基　　金：黑龙江省教育厅青年学术骨干支持计划项目(1251G049)

摘　　要：大豆乳清废水中含有高浓度的BOD5和COD,难生物降解,且易发生腐败。为提高大豆乳清废水的可生物降解性,采用固定化木瓜蛋白酶水解大豆乳清废水,并研究最佳工艺条件。固定化工艺中以戊二醛为交联剂,壳聚糖为载体。固定化木瓜蛋白酶水解大豆乳清废水的最佳工艺条件为:在55℃、pH为8.0、加酶量为3%的条件下,水解2.5 h后,底物水解度可达36.48%。废水中BOD5/COD比值可达0.7以上,废水的可生化性程度显著提高,对固定化酶进行回收,酶活力回收率可达97%,可以有效重复使用。The soybean whey wastewater contains high concentration of BOD5 and COD. It is hard to biodegraded and easy to decay. The purpose of the study is to define the proper process of soybean isolated protein hydrolyzed by immobilized papain enzyme. Glutaraldehyde was used as cross linking agent, and chitosan was used as carder in this experiment. The results showed that the optimal hydrolyzing conditions of immobilized papain enzyme was as follows： 55 ~C, pH 8.0, enzyme dosage was 3%, after 2.5 hours hydrolysis, protein hydrolysis degree could be 36.48%. Ratio of BOD5 to COD was above 0.7. Biodegradability of soybean isolated protein whey wastewater could be improved. The immobilized enzyme could be reused. The recovered immobilized enzyme activity was 97% of raw immobilized enzyme.

关 键 词：固定化 木瓜蛋白酶 壳聚糖 大豆乳清废水 

分 类 号：S767.5[农业科学—森林保护学]