^(60)Co-γ射线辐照诱变筛选纳豆激酶高活耐热菌株  

Screening of Strains with the High Activity and Thermostability Nattokinase by ^(60)Co γ-ray Irradiation

作　　者：李淑英[1] 聂莹[1] 杜欢[1] 赵仲麟[2] 马鑫[3] 李燕[4] 唐选明[1] 

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]河南农业大学理学院,河南郑州450002 [3]中国农业科学院信息研究所,北京100081 [4]郑州轻工业学院食品与生物工程学院,河南郑州450002

出　　处：《核农学报》2013年第6期782-785,共4页Journal of Nuclear Agricultural Sciences

基　　金：中国农业科学院科技经费项目(201211)

摘　　要：为得到高活热稳定性的纳豆激酶生产菌,本研究采用60Co-γ射线辐照诱变纳豆激酶生产菌,以酪蛋白平板法作为活性筛选方法。经过辐照剂量筛选确认800 Gy为最适诱变剂量,在该剂量下正突变率最大,高达45%。经过800 Gy诱变共得到60株活性提高的菌株。不同温度热处理后,11株菌在65℃显示了很好的热稳定性。In this study,Bacillus natto was irradiated by 60Co γ-ray,and activity was determined by Casein plate method in order to get high activity and thermostability strains.60 strains with high activity were obtained through irradiation by 800 Gy 60Co γ-ray.In this dose,the positive mutation rate was 45%.Then 60 strains was treated by different tempreture and 11 strains showed thermostability at 65℃.

关 键 词：纳豆激酶 枯草芽孢杆菌 高活性 热稳定性 

分 类 号：TS201.3[轻工技术与工程—食品科学]