响应面法优化林蛙蛙皮中蛋白质的提取工艺  

Optimization of Extracting Conditions for Protein from Frog Skin(Rana Chensinensis) by Response Surface Methodology

作　　者：侯召华[1] 孙晓东[1] 宁浩然[1] 张宇[1] 金春爱[1] 崔松焕[1] 

机构地区：[1]中国农业科学院特产研究所,吉林长春130112

出　　处：《安徽农业科学》2013年第12期5362-5365,共4页Journal of Anhui Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(2012ZL090)

摘　　要：[目的]利用响应面分析法优化林蛙皮中蛋白质的提取工艺。[方法]通过单因素试验,确定响应面试验因素与中心水平。根据Box-Behnken试验设计原理采用4因素3水平的响应面法,依据回归分析,以蛋白质提取率为响应值作响应面,分析各因素的显著性和交互作用。[结果]林蛙皮中蛋白质提取的最佳条件为:液料比30.35,提取时间3.28 h,提取温度11.13℃,pH为6.01,提取次数为2次。在最佳工艺条件下,蛋白质的实际提取率为6.11%。利用SDS-PAGE得到林蛙皮蛋白质分子量主要集中在43～20 kD。[Objective] The technology for extracting protein from frog skin was optimized by response surface methodology（RSM）.[Method] The RSM experiment factors and levels were determined by single factor experiments.On the basis of Box-Behnken experiment design,a 4 factors and 3 levels’RSM was applied to get the RSM plot with the protein extraction rate as the response value after regression analysis.Then the significance and interaction of every factor were analyzed.[Result]The optimum extracting conditions of protein were as follows： the ratio of water to material of 30.35,extracting time of 3.28 h,extracting temperature of 11.13 ℃,pH of 6.01,2 times.The actual extraction rate is 6.11% under the optimum conditions.Using SDS-PAGE,the main range of molecular weight of protein is 43-20 kD.

关 键 词：林蛙皮 蛋白质 响应面法 提取 

分 类 号：S966.39[农业科学—水产养殖]