产广谱细菌素乳酸菌CW3的筛选和初步鉴定  

Screen and Preliminary Identification of Lactic acid bacteria to Produce Broad-Spectrum Bacteriocin

作　　者：吕好新[1] 王巍东[2] 谈重芳[1] 杨飞飞[1] 焦迎春[3] 王雁萍[1] 李宗伟[1] 

机构地区：[1]郑州大学河南省离子束生物工程重点实验室,河南郑州450052 [2]新乡医学院第三附属医院,河南新乡453003 [3]青海大学生物科学系,青海西宁810016

出　　处：《安徽农业科学》2013年第12期5504-5504,5565,共2页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(41101244);河南省基础和前沿研究项目(122300410161);河南省教育厅自然科学研究计划项目(2010B180029)

摘　　要：[目的]对CW3菌株进行筛选,并且进行初步鉴定。[方法]首先采用牛津杯双层平板法进行产广谱细菌素菌株的初筛,再将初筛得到的菌株进行排除酸和过氧化氢干扰,并检测抑菌物质的蛋白质性质,最终对复筛得到的菌株进行鉴定。[结果]试验得出,排除有机酸、过氧化氢等干扰因素后,发酵液仍有抑菌作用;用胰蛋白酶和胃蛋白酶处理后,发酵液抑菌活性急剧下降,确定产生的抑菌物质具有蛋白质性质,是一类细菌素。经过生理生化试验初步鉴定菌株CW3为植物乳杆菌。[结论]菌株CW3是一种能产广谱细菌素的植物乳杆菌。[Objective] CW3 strain was screened and preliminarily identified.[Method]Oxford cup double plate method was adopted to primarily screen spectrum bacteriocin,then excluding acid and hydrogen peroxide disturbance,the protein property of anti-bacteria material was detected,the strain of rescreening was identified.[Result]The supernatant of CW3 strain can inhibit the growth of indicator strains excluded hydrogen peroxide and organic acid.The inhibitive activity decreased largely after treatment with trpsin and pepsin,which can draw that the anti-microbial substances were bacteriocin.The results of identification of physiology and biochemistry preliminarily identified that CW3 strain was a Lactobacillus plantarum.[Conclusion] CW3 strain was a Lactobacillus plantarum,which can generate spectrum bacteriocin.

关 键 词：细菌素 牛津杯双层平板法 植物乳杆菌 

分 类 号：S609.9[农业科学—园艺学]