海参肽的抗氧化活性与分子量的相关性研究  

The relationship between antioxidant activity of sea cucumber peptide and its molecular weight

作　　者：付学军[1] 金海珠[1] 

机构地区：[1]烟台大学生命科学学院,烟台264005

出　　处：《食品科技》2013年第7期63-66,共4页Food Science and Technology

基　　金：山东省自然科学基金项目(Y2008D42)

摘　　要：以新鲜海参为材料,酶解后分别用5ku和10ku的超滤膜超滤,得到Mr>10ku、5ku<Mr≤10ku和Mr≤5ku的海参肽溶液,又对Mr≤5ku的海参肽进行分子筛层析,分离出2个组分,分别测定5个不同分子量组分的抗氧化性。结果表明,不同分子量的海参肽清除超氧阴离子的活性差别不大,Mr≤5ku的海参肽清除超氧阴离子活性最高;而不同分子量段海参肽清除羟自由基活性存在较大差异,Mr≤5ku的分子筛层析组分二和Mr>10ku的海参肽具有较高的清除羟自由基活性。In this paper, sea cucumber peptides with molecular weight （Mr）10 ku, 5 kuMr≤10 ku and Mr≤5 ku were made from fresh sea cucumber by enzymed, ultra filtrated with 5 ku and 10 ku of ultra filtration membrane. The peptides with Mr≤5 ku were separated into two factions by molecular sieve chromatography, The antioxidation stability of these five peptides with different molecular weight were determined. The results showed that different molecular weight peptides of sea cucumber have little different on activity of superoxide free radical clearance, The peptide with Mr≤5 ku has the highest activity of removment superoxide free radical. And different molecular weight peptides of sea cucumber have bigger different activity in hydroxy free radical clearance. The second faction of molecular sieve chromatography of peptides with Mr≤5 ku and peptides with Mr10 ku has the higher activity of hydroxy free radical clearance.

关 键 词：海参肽 抗氧化 自由基清除率 分子量 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]