Lentivirus carrying the Atoh1 gene infects normal rat cochlea  

作　　者：Song Pan Jingzhi Wan Shaosheng Liu Song Zhang Hao Xiong Jun Zhou Wu Xiong Kunfei Yu Yong Fu 

机构地区：[1]Department of Otolaryngology, Guangming New District People's Hospital [2]Department of Surgery, Hubei University of Science and Technology [3]Department of Otolaryngology, Beilun Branch of the First Affiliated Hospital, College of Medicine, Zhejiang University [4]Department of Otolaryngology, Second Affiliated Hospital, Sun Yat-sen University [5]Department of Otolaryngology, First Affiliated Hospital, College of Medicine, Zhejiang University

出　　处：《Neural Regeneration Research》2013年第17期1551-1559,共9页中国神经再生研究（英文版）

基　　金：supported by grants from the National Basic Research Program of China (973 Program), No. 2012CB967900, 2012CB967904;the National Natural Science Foundation of China, No. 81070782;the Natural Science Foundation of Zhejiang Province, China, No. 30672308;the Qianjiang Talent Project of Science and Technology Ministry in Zhejiang Province, No. 2011R10014;the Natural Science Foundation of Ningbo, No. 2011A610042

摘　　要：Lentivirus carrying the Atohl gene can infect Corti＇s organ and express a hair-like cell surface marker in the supporting cell area. However, expression of the gene carried by adenovirus is instantaneous, which undoubtedly limits its clinical application. Lentivirus acts as a carrier that can stably and continuously express genes. In this study, the cochlear structure and hearing level were not affected, and Atohl gene carried by lentivirus promoted the production of hair-like cells in the cochlear supporting cell area. This led to expression of the hair-like cell surface marker myosin 7a 30 days after lentivirus carrying Atohl was microinjected into the cochlear round window of rats.Lentivirus carrying the Atohl gene can infect Corti＇s organ and express a hair-like cell surface marker in the supporting cell area. However, expression of the gene carried by adenovirus is instantaneous, which undoubtedly limits its clinical application. Lentivirus acts as a carrier that can stably and continuously express genes. In this study, the cochlear structure and hearing level were not affected, and Atohl gene carried by lentivirus promoted the production of hair-like cells in the cochlear supporting cell area. This led to expression of the hair-like cell surface marker myosin 7a 30 days after lentivirus carrying Atohl was microinjected into the cochlear round window of rats.

关 键 词：neural regeneration gene therapy Atohl LENTIVIRUS hair cells myosin 7a COCHLEA auditorythreshold grants-supported paper NEUROREGENERATION 

分 类 号：R764[医药卫生—耳鼻咽喉科]