Neuropeptide Y gene transfection inhibits post-epileptic hippocampal synaptic reconstruction  

作　　者：Fan Zhang Wenqing Zhao Wenling Li Changzheng Dong Xinying Zhang Jiang Wu Na Li Chuandong Liang 

机构地区：[1]Graduate School, Hebei Medical University [2]Hebei General Hospital [3]Department of Neurosurgery, People's Hospital of Hebei Province

出　　处：《Neural Regeneration Research》2013年第17期1597-1605,共9页中国神经再生研究（英文版）

摘　　要：Exogenous neuropeptide Y has antiepileptic effects; however, the underlying mechanism and optimal administration method for neuropeptide Y are still unresolved. Previous studies have used intracerebroventricular injection of neuropeptide Y into animal models of epilepsy. In this study, a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene was injected into the lateral ventricle of rats, while the ipsilateral hippocampus was injected with kainic acid to establish the epileptic model. After transfection of neuropeptide Y gene, mossy fiber sprouting in the hippocampal CA3 region of epileptic rats was significantly suppressed, hippocampal synaptophysin （p38） mRNA and protein expression were inhibited, and epileptic seizures were reduced. These experimental findings indicate that a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene reduces mossy fiber sprouting and inhibits abnormal synaptophysin expression, thereby suppressing post-epileptic synaptic reconstruction.Exogenous neuropeptide Y has antiepileptic effects; however, the underlying mechanism and optimal administration method for neuropeptide Y are still unresolved. Previous studies have used intracerebroventricular injection of neuropeptide Y into animal models of epilepsy. In this study, a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene was injected into the lateral ventricle of rats, while the ipsilateral hippocampus was injected with kainic acid to establish the epileptic model. After transfection of neuropeptide Y gene, mossy fiber sprouting in the hippocampal CA3 region of epileptic rats was significantly suppressed, hippocampal synaptophysin （p38） mRNA and protein expression were inhibited, and epileptic seizures were reduced. These experimental findings indicate that a recombinant adeno-associated virus expression vector carrying the neuropeptide Y gene reduces mossy fiber sprouting and inhibits abnormal synaptophysin expression, thereby suppressing post-epileptic synaptic reconstruction.

关 键 词：neural regeneration gene therapy neural plasticity NEURODEGENERATION recombinantadeno-associated virus vector neuropeptide Y epilepsy kainic acid synaptic remodeling mossyfiber sprouting hippocampus SYNAPTOPHYSIN NEUROREGENERATION 

分 类 号：R742.1[医药卫生—神经病学与精神病学]