双波长分光光度法测定玉米微孔淀粉的直链淀粉、支链淀粉含量  

Determination of Amylose and Amylopectin in Micro-Pore Starch of Grain by Dual-Wavelength Spectrophotometry

作　　者：周琼[1] 

机构地区：[1]安康学院化学化工系,陕西省安康市育才路92号725000

出　　处：《光谱实验室》2013年第4期1569-1572,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：采用双波长分光光度法同时测定玉米和玉米微孔淀粉中直链和支链淀粉的含量。由直链淀粉-碘、支链淀粉-碘复合物的吸收光谱,确定直链和支链淀粉的最大波长分别为607、534nm,选取参比波长分别为433、729nm,将样品的吸光度差值代入回归方程,可求出样品中直链和支链淀粉的含量。通过测定玉米淀粉的直链、支链含量,以期为微孔淀粉的致孔机理研究提供理论参考。The paper mainly uses dual-wave length spectrophotometry method to study the determine-nation of amylose and amylopectin in micro-pore starch of grain.Based on the absorption spectra of the two complexes,the amylose-wave length of 433/607nm and amylopectin wave length of 534/729nm were obtained as the measuring wave length.According to the linear regression equationes,the paper can determine the content of amylose and amylopectin in sample solution.It can provide the data for study the mechanism of making pore of starch.

关 键 词：双波长分光光度法 直链淀粉 支链淀粉 玉米微孔淀粉 

分 类 号：O657.32[理学—分析化学]