产蛋白酶海洋微生物的筛选鉴定及发酵  

Screening and identification of protease-producing marine microorganism and its fermentation conditions for alkaline protease production

作　　者：崔少飞[1,2] 董超[2] 史延茂[2] 刘丹[1,2] 崔小颖[1] 

机构地区：[1]河北工业大学化工学院,天津300130 [2]河北省科学院生物研究所,河北石家庄050081

出　　处：《中国酿造》2013年第6期110-113,121,共5页China Brewing

摘　　要：从秦皇岛附近海域水样中筛选出了1株蛋白酶生产菌,并对其发酵条件进行了初步研究。经16S rDNA鉴定此菌株属假交替单胞菌属(Pseudoalteromonas sp.),所产蛋白酶为中性蛋白酶。液体培养基为人工海水,蛋白胨10.0g/L,酵母浸粉5.0g/L,葡萄糖5.0g/L,硝酸铵1.0g/L,无水氯化钙1.0g/L,在33℃,初始pH值6.0,50mL装液量,200r/min条件下发酵,发酵液酶活可达1140 U/mL。A strain of protease-producing marine microorganism was isolated from the sea-water in Qihuangdao.The marine microorganism was identified belonging to the genus of Pseudoalteromonas sp.,and the protease which produced by the strain was neutral.The optimum broth was consisted of peptone 10.0g/L,yeast extract powder 5.0g/L,glucose 5.0g/L,NH4NO3 1.0g/L,CaCl2 1.0g/L.Enzyme activity could reach 1140U/ml under the fermentation condition： fermentation temperature 33℃,initial pH 6.0,filling volume 50 ml,rotational speed 200 r/min.

关 键 词：海洋微生物 蛋白酶 菌种筛选 

分 类 号：Q939.9[生物学—微生物学]