榆耳发酵培养基及发酵条件的优化  

Optimization of medial components and cultural conditions gloeostereum incarnatum

作　　者：崔京春[1,2] 郭海勇[3] 邢效瑞[1,4] 吴俊罡 关博[1] 胡文忠[1] 钱爱东[4] 李玉[2] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600 [2]吉林农业大学食药用菌教育部工程研究中心,吉林长春130118 [3]吉林师范大学生命科学学院,吉林四平136000 [4]吉林农业大学动物科学技术学院,吉林长春130118 [5]大连吉翔农业科技有限公司,辽宁大连116620

出　　处：《食品工业科技》2013年第15期131-135,共5页Science and Technology of Food Industry

基　　金：2011年度教育部"长江学者和创新团队发展计划"创新团队项目(IRT1134);吉林省教育厅"十二五"科学技术研究项目(吉教科合字2012178);大连民族学院博士启动金(20116205)

摘　　要：系统考察了培养基成分及发酵条件对榆耳JX2010菌株液体发酵生物量和发酵液抑菌活性的影响。培养基单因素筛选试验结果表明,玉米淀粉和黄豆粉为最适碳源和氮源;采用均匀设计法对培养基组成优化结果表明,最佳的培养基配方为4.6%玉米淀粉、1.6%黄豆粉、0.03%硫酸镁、0.03%磷酸二氢钾、0.01%磷酸氢二钾、0.001%硫酸锌、0.001%硫酸锰及0.1g/L维生素B1;以此优化的培养基对培养条件研究的结果表明,在10%接种量、20%摇瓶装液量、培养基初始pH5.5的条件下发酵培养时间7d时,发酵效果最好,发酵生物量达到(14.98±0.74)g/L,抑菌直径达到(16.7±0.5)mm。The effects of medial component and cultural conditions on biomass and antibacterial activity of fermented Gloeostereum incarnatum JX2010 strain were studied.The results of medium single factor screening tests showed that the best carbon source and nitrogen source were corn starch and soybean powder respectively;the best formula of culture medium were 4.6% corn starch,1.6% soybean powder,0.03% MgSO4,0.03% KH2PO4, 0.01% K2HPO4,0.001% ZnSO4 0.001% MnSO4 and 0.1mg/L VB, by Uniform design method. Culturing in this optimized medium for 7d with 10% inoculums size,20% of flask liquid volume and 5.5 of initial pH of the culture medium, the highest biomass of the fermented Gloeostereum incamatum was （ 14.98 ±0.74） g/L and the bacteriostatic diameter was（ 162±0.5 ）mm.

关 键 词：榆耳 培养条件优化 均匀设计 生物量 抑菌活性 

分 类 号：TQ929[轻工技术与工程—发酵工程]