三种不同原料发酵丁醇的比较  

Comparison of Three Different Materials Fermented Butanol

作　　者：张霞[1,2] 杨旭 马晓建[1] 

机构地区：[1]郑州大学化工与能源学院,河南郑州450001 [2]华北水利水电学院,河南郑州450011 [3]郑州凯乐生物能有限公司,河南郑州450001

出　　处：《生物技术》2013年第3期93-97,共5页Biotechnology

基　　金：国家"十一五"科技支撑计划项目(2007BAD66B04)资助

摘　　要：目的:为了比较不同生物质发酵丁醇的差异。方法:分别以8%玉米粉、米糠、汽爆玉米秸秆为原料,丙酮丁醇梭菌(Clostridium acetobutylicum zzu-2)为发酵菌,37℃厌氧发酵生产丁醇。并考察了初始pH、秸秆酶解液上清浓缩液对丁醇产量的影响。结果:8%玉米粉、米糠、汽爆玉米秸秆的发酵后丁醇浓度分别为13.45g/l、13.17g/l和9.64g/l。总溶剂浓度分别为21.19g/l、20.64g/l和14.88g/l。分批发酵时间分别为36h、64h和68h。结论:丙酮丁醇梭菌pH适应范围为4.4～6.5,具有淀粉酶的活性,不具备纤维素的活性。与玉米粉和米糠相比,汽爆玉米秸秆酶解后糖浓度较低,为57.5g/l,副产物较多,导致总溶剂和丁醇浓度偏低,分别为14.88g/l、9.64g/l。秸秆酶解液的糖浓度亟待提高。Objective： In order to compare the diffrrence of different biomass fermented butanol. Method： Butanol was anaerobic fermented by Clostridium acetobutylicum zzu - 2 at 37 ℃,respectively with 8% corn flour, rice bran, steam -exploded corn stover as raw materi- al. And the effects of the initial pH, concentrated steam - exploded corn stover enzymatic hydrolysis liquid on butanol yield were also stud- ied. Result：Butanol concentration respectively was 13.45 g/l, 13.17 g/1 and 9. 64 g/1. General solvent concentration respectively was 21.19 g/l,20. 64 g/1 and 14. 88 g/1. Fermentation time respectively was 36 h and 64 h and 68 h. Conclusion：Clostridium acetobutylicum zzu - 2 has the wider pH value range of 4. 4 - 6. 5. And it has the activity of amylase, has not the activity of cellulose. Compared with corn flour and rice bran, sugar content （57. 5g/l） of steam - exploded corn stover after enzymatic hydrolysis was low, the by - products were more,which led to lower concentrations of the solvent and butanol（ 14. 88g/l and 9. 64g/l）. The sugar concentration of corn stover enzy- molysis liquid needs to be improved.

关 键 词：米糠 秸秆 丁醇 发酵 酶解液 

分 类 号：TQ353.2[化学工程]