Ataxin-3相互作用蛋白A3IP的克隆与细胞及组织定位的初步研究  

Cloning and localization of A31P - a novel protein that interacts with ataxin-3

作　　者：黄凤珍[1] 侯漩[1] 王果[3] 蔡芳[3] 房海燕[3] 潘乾[3] 夏昆[3] 唐北沙[2,3] 江泓[1,2] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008 [2]中南大学神经退行性疾病研究中心 [3]中国医学遗传学国家重点实验室

出　　处：《中华医学遗传学杂志》2013年第4期394-398,共5页Chinese Journal of Medical Genetics

基　　金：国家重点基础研究计划（973项目）（2012CB944601,2012CB517902,2011CB510002）,教育部新世纪优秀人才支持计划（NCET-10-0836）,国家自然科学基金（81271260,30971585,30871354,30710303061,30400262）,中南大学大学生创新项目（CY12400）

摘　　要：目的根据前期筛选到的与ataxin-3蛋白羧基端相互作用的A3IP部分序列，进行A3伊基因的克隆，并探讨其细胞及组织定位情况。方法应用生物信息学及Northern印迹方法克隆A3IP基因并检测其转录本在人体组织和人脑不同部位的表达情况；应用Western印迹及免疫荧光方法检测A3IP蛋白在细胞中的表达及定位情况；应用免疫组织化学染色方法检测A3IP蛋白在人体各组织和人脑不同部位的表达及定位情况。结果对A3IP基因序列全长阅读框进行了cDNA克隆及序列拼接；检测了A3IP的3个转录本（1kb、1．35kb、6kb）在人体不同组织和人脑不同部位的表达谱；获得了A3IP-pEGFP在COS-7细胞和人大脑皮质、小脑、肌肉、周围神经、肝脏、肾脏的表达及定位情况。结论所克隆的AMP基因编码ataxin-3的一种相互作用蛋白A3IP，其3个剪切本在人体多种组织和人脑不同部位广泛表达，是一种细胞胞浆蛋白。Objective To clone an A3IP gene and investigate its cellular and histological localization based on previous research which has identified part of A3IP sequence interacting with carboxyl-terminal of ataxin-3. Methods Bioinformatic and Northern blotting were applied to clone the A3IP gene and detect the expression of its transcripts in various human tissues and brain regions. Western blotting and immunofluorescence staining were applied to detect expression of A3IP protein in cultured cells. Immunohistochemistry staining was applied to study the expression of A3IP protein in various human tissues and brain regions. Results cDNA cloning of A3IP gene＇s reading frame and its sequence assembly were completed. Three transcripts （1 kb, 1.35 kb and 6 kb, respectively） of A3IP were found to express in various human tissues and brain regions. A3IP-pEGFP expresses in cytoplasm of cultured COS-7 cells and various human tissues and brain regions including cerebral cortex, cerebellum, muscle, peripheral nerve, liver and kidney. Conclusion The cloned A3IP gene encodes A3IP, a novel ataxin-3-interacting protein. Three transcripts of A3IP are expressed in various human tissues and brain regions. A3IP is a cytosolic protein.

关 键 词：Ataxin-3蛋白 相互作用蛋白 A3IP基因 基因克隆 细胞和组织定位 

分 类 号：R744.7[医药卫生—神经病学与精神病学]