脑微血管内皮细胞TNFR差异表达对rhTNF开放血肿瘤屏障的影响  

Effect of TNFR differentially expressed on brain microvascular endothelial cells for rhTNF open the blood-tumor barrier

作　　者：秦丽娟[1] 王健[1] 谷艳婷[2] 张田[1] 王艳蕾[1] 薛一雪[3] 

机构地区：[1]河北联合大学基础医学院,河北唐山063000 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [3]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001

出　　处：《中风与神经疾病杂志》2013年第7期580-582,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No:81101912);河北省卫生厅科研基金项目(No.20120144);河北联合大学博士启动基金项目(No.BS09012);河北联合大学大学生创新性实验计划项目(X2012011);国家教育部新教师基金项目(No.20102134120007);辽宁省博士启动基金项目(No.20101109)

摘　　要：目的观察脑微血管内皮细胞的肿瘤坏死因子受体(TNFR)差异表达对rhTNF开放血肿瘤屏障的影响。方法建立TNFR1/TNFR2高表达的脑微血管内皮细胞株,并与C6胶质瘤细胞一起通过Transwell小室构建体外血肿瘤屏障模型。免疫荧光技术检测脑微血管内皮细胞TNFR的表达水平。荧光分析法测定给予rhTNF后的血肿瘤屏障通透性变化。结果脑微血管内皮细胞的TNFR1高表达时,rhTNF开放血肿瘤屏障的程度最大。而TNFR2高表达时,血肿瘤屏障开放程度与对照组相比无明显差异。结论 rhTNF可能是通过与脑微血管内皮细胞的TNFR1结合而引起血肿瘤屏障开放的。Objective To observe effect of TNFR differentially expressed on brain microvascular endothelial cells for rhTNF open the blood-tumor barrier.Methods Brain microvascular endothelial cell（BMEC） of TNFR1/TNFR2 highly expression line was established and blood tumor barrier in vitro model with C6 glioma cells through Transwell was built.Using immunofluorescence to detect TNFR expression levels in brain microvascular endothelial cells.Fluorescence analysis method was used to determine blood tumor barrier permeability changes after given the rhTNF.Results Blood-tumor barrier openess reached maximum by rhTNF when TNFR1 high expression on brain microvascular endothelial cells,but high expression of TNFR2 did not cause significant blood-tumor barrier openness than control group.Conclusion rhTNF may combine TNFR1 on brain microvascular endothelial cells and open the blood tumor barrier.

关 键 词：胶质瘤 血肿瘤屏障 TNFR 

分 类 号：R739.41[医药卫生—肿瘤]