生物毒性测试微孔板MTOXPlate的分析性能研究  

Analysis Performance of Biological Toxicity Testing MTOXPlate Using Microplate

作　　者：林奕云[1,2,3,4] 张国霞[1,3,4] 贺丽苹[2] 孙国萍[1,3,4] 许玫英[1,3,4] 

机构地区：[1]广东省微生物研究所广东省菌种保藏与应用重点实验室,广州510070 [2]华南农业大学食品科学学院,广州510642 [3]广东省微生物应用新技术公共实验室,广州510070 [4]广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地,广州510070

出　　处：《环境科学》2013年第8期3294-3302,共9页Environmental Science

基　　金：国家自然科学基金委-广东联合基金项目(U1133005);广东省产学研项目(2011A090100030);广东省自然科学基金研究团队项目(9351007002000001);国家水体污染控制与治理科技重大专项(2008ZX07211-007);广东省科技攻关项目(2011B060100007);广东省科学院青年基金项目(qnjjsq201109)

摘　　要：采用以11种微生物为指示生物的毒性测试微孔板MTOXPlate,以污染物对微生物细胞的脱氢酶活性抑制为测试终点,对重金属Hg2+、Cd2+、Cr6+、Pb2+,酚类化合物2,4-二氯酚(2,4-DCP)、邻氯苯酚(2-CP)、对氯苯酚(4-CP)、邻硝基酚(2-NP)、对硝基酚(4-NP)及地表水、生活污水、印染废水的生物毒性进行分析.结果表明,MTOXPlate生物毒性测试微孔板具有良好的毒性分析性能,Hg2+、Cd2+、Cr6+和Pb2+的EC50值分别为0.617、21.05、35.21和11.22 mg·L-1,2,4-DCP、2-CP、4-CP、2-NP和4-NP的EC50值分别为27.26、64.29、44.19、85.89和33.84 mg·L-1.MTOXPlate分析得到地表水原水的抑制率均小于20%,生活污水的EC50值为62.19%,印染废水的EC50值为16.42%,表明生物毒性测试微孔板MTOXPlate能真实地反映地表水、生活污水和印染废水的毒性情况.Biological toxicities of heavy metals（Hg2＋,Cd2＋,Cr6＋ and Pb2＋）,phenolic compounds（2,4-DCP,2-CP,4-CP,2-NP and 4-NP）,surface water and wastewater were determined with the MTOXPlate based on 11 genetically diverse microorganisms and the analysis endpoint of dehydrogenase activity inhibition.The results showed that the MTOXPlate had good performance for toxicity analysis of heavy metals and phenolic compounds.The EC50 values of Hg2＋,Cd2＋,Cr6＋,Pb2＋,2,4-DCP,2-CP,4-CP,2-NP and 4-NP detected by the MTOXPlate were 0.617,21.05,35.21,11.22,27.26,64.29,44.19,85.89 and 33.84 mg·L-1,respectively.The dehydrogenase activity inhibition of surface water was less than 20%.Moreover,the EC50 values of sewage and dyeing wastewater were 62.19% and 16.42%,respectively.Thus,the biological toxicity testing technology MTOXPlate could reflect the actual toxicity of surface water,sewage and dyeing wastewater.

关 键 词：MTOXPlate 生物毒性 重金属 酚类化合物 污水 

分 类 号：X171.5[环境科学与工程—环境科学]