高效液相色谱法检测月饼中丙烯酰胺的含量  

Determination of Acrylamide in Moon Cake by High Performance Liquid Chromatography

作　　者：陆文蔚[1] 黄玥[1] 艾清[1] 白晨[1] 

机构地区：[1]上海商学院食品系,上海200234

出　　处：《食品研究与开发》2013年第13期92-95,共4页Food Research and Development

基　　金：上海商学院校级教改项目(编号:253110);中央财政支持地方高校发展专项资金项目(编号:YC1105-D-4-1)

摘　　要：研究了高效液相色谱测定月饼中丙烯酰胺的方法。采用Inertsil ODS-SP(250 mm×46 mm)色谱柱,以210 nm为检测波长,以5%乙腈水溶液作为流动相,流速为0.8 mL/min,柱温25℃,进样量20μL。该方法的最低检测限为0.124μg/kg。丙烯酰胺在0.1 mg/L^1.0 mg/L范围内呈良好的线性关系,回收率大于80%。结果显示:固相萃取与溶剂萃取都适合作为样品预处理方法,适合这类焙烤食品的检测。The method of high performance liquid chromatography for acrylamide determination in moon cake was established. The acrylamide was separated on a C18 column （Inertsil ODS-SP,250 mmx46 mm） with 5 % acetonitrile-water solution as mobile phase, and detected at 210 nm. The limit of detection is 0.124ug/kg. The linear plots were obtained were obtained between 0.1 mg/L-1.0 mg/L. Overall recoveries were higher than 80 % and RSD value were between 0.17 % and 4.33 %. The experiment results showed that solid phase extraction and solvent extraction were suitable for analysis of acrylamide in moon cake.

关 键 词：丙烯酰胺 月饼 高效液相色谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]