辣木茎叶中水溶性多糖的提取及抗氧化活性的研究  

Study on Extraction and Antioxidant Activity of Water-soluble Polysaccharides from Moringa Oleifera

作　　者：梁鹏[1,2] 甄润英[1,2] 

机构地区：[1]天津农学院食品科学系,天津300384 [2]天津市农副产品深加工技术工程中心,天津300384

出　　处：《食品研究与开发》2013年第14期25-29,共5页Food Research and Development

摘　　要：主要探讨辣木茎叶中水溶性多糖的提取工艺条件以及抗氧化活性。以辣木茎叶干粉为原料,采用水为提取剂,通过单因素和正交试验对浸提温度、浸提时间及料液比进行研究;采用水杨酸法和邻苯三酚法分别测定辣木多糖对羟自由基以及超氧阴离子的清除率,以确定提取物的抗氧化活性。实验条件下辣木茎叶多糖最佳提取工艺条件为料液比1∶20(g/mL)、浸提温度80℃、浸提时间120 min,在此条件下,辣木粗多糖的提取率可达5.66%;多糖提取物对羟自由基及超氧阴离子均有清除作用,且随着提取物浓度的提高对二者的清除作用逐渐增强,存在剂量效应关系。清除作用的半数抑制率(IC50)分别是7.252 8 mg/mL和2.501 1 mg/mL。In order to explore the extraction conditions of water-soluble polysaccharide from the Moringa oleifera leaves and antioxidant activity of it,dry powder was used as raw materials and using water as extracting solvent to extract polysaccharide from Moringa oleifera.The extraction temperature,time and solid-liquid ratio were optimized by orthogonal experiments.Then,use methods of Salicylic acid and Pyrogallol to test antioxidant activity on polysaccharide from Moringa oleifera in different concentrations respectively.The results showed that the extraction rate was up to 5.66 %,the optimal extraction conditions was extract 120 min,using 80 ℃ hot water at a solid-liquid ratio of 1∶20（g/mL）.The polysaccharides from Moringa oleifera leaf had a certain degree of antioxidant activities in vitro： scavenge OH.oand O2-in a dose-dependant manner with the IC50of 7.252 8 mg/ml and 2.501 1 mg/ml respectively.

关 键 词：辣木 多糖 提取工艺 抗氧化活性 

分 类 号：TQ914[化学工程]