响应面法优化富铬灵芝发酵培养基  

Optimization of Fermentation Medium for Chromium-enriched Ganoderma lucidum by Response Surface Methodology

作　　者：张帅[1] 董基[1] 黄志明[1] 

机构地区：[1]肇庆学院化学化工学院,广东肇庆526061

出　　处：《食品科学》2013年第15期208-212,共5页Food Science

摘　　要：通过单因素试验确定富铬灵芝发酵培养基中CrCl3.6H2O添加量的适宜范围,并由Plackett-Burman设计筛选出对灵芝菌体铬总含量影响显著的3种培养基组分:CrCl3.6H2O、黄豆饼粉和玉米粉。继而采用最陡爬坡试验逼近最大响应区域后,通过中心组合设计的响应面试验建立以铬总含量为响应值的二次回归模型,最终得到最优发酵培养基组成为:CrCl3.6H2O 250.5mg/L、黄豆饼粉13.07g/L、玉米粉27.35g/L、蔗糖18g/L、VB10.012g/L、NH4Cl1.6g/L、MgSO4.7H2O 0.8g/L、KH2PO40.8g/L,在此条件下测得铬总含量为1525.92μg/L,与预测值1530.21μg/L非常接近,说明预测模型适用、可靠,可应用于富铬灵芝发酵培养基的优化。In the current study, the appropriate concentration range of CrCl3·6H2O in the fermentation medium for chromium- enriched Ganoderma lueidum was established by one-factor-at-a-time design. CrCl3·6H2O, soybean cake powder and corn meal were identified by Plackett-Burman （PB） design as main medium components that influence the total chromium content of G. lueidum mycelia and the steepest ascent method was used to approach the neighborhood of the optimum. A quadratic regression model with the total chromium content of G. lucidum mycelia as the dependent variable was established by central composite design （CCD）. The optimum fermentation medium composition was determined by response surface analysis as 250.5 mg/L CrCl3·6H2O, 13.07 g/L soybean cake powder, 27.35 g/L corn meal, 18 g/L sucrose, 0.012 g/L VBI, 1.6 g/L NH4Cl, 0.8 g/L MgSO4·7H2O and 0.8 g/L KH2PO4. The measured value of total chromium content was 1525.92 μg/L under the optimal conditions, which was well consistent with the predicted value of 1530.21 μg/L. Hence the prediction model was available and reliable for the optimization of fermentation medium for chromium-enriched G. lucidum.

关 键 词：富铬灵芝 发酵培养基 优化 PLACKETT-BURMAN设计 中心组合设计 响应面法 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]