4种常见海参对小鼠肠道黏膜免疫功能的保护作用  

Protective Effect of Common Species of Sea Cucumbers on Intestinal Mucosal Immunity in Mice

作　　者：李学敏[1] 于卿 郑荣[1] 左涛[1] 曹斌斌[1] 唐庆娟[1] 王静凤[1] 王玉明[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]济宁市农业委员会,山东济宁272019

出　　处：《食品科学》2013年第15期240-245,共6页Food Science

基　　金：国家自然科学基金项目(31071525;31101281);"泰山学者"建设工程专项;教育部"长江学者和创新团队发展计划"项目

摘　　要：目的:研究4种常见海参对环磷酰胺所致小鼠肠黏膜损伤的保护作用,并初步探讨其保护机制。方法:70只BALB/c小鼠随机分为7组:正常组、模型组、阳性对照组、仿刺参组、美国肉参组、梅花参组、海地瓜组。采用腹腔注射环磷酰胺构建肠道黏膜损伤小鼠模型。记录小鼠体质量变化情况,检测胸腺、脾指数,制备小肠切片并进行组织学观察,统计绒毛(villus)长度、隐窝(crypt)深度并统计两者比值(V/C),酶联免疫法(ELISA)检测肠黏膜SIgA、IFN-γ、IL-4的含量并统计黏膜IFN-γ/IL-4,免疫印迹法(Western blotting)检测小肠IgA的蛋白表达量。结果:与模型组相比,4种海参均可显著提高小鼠脾指数,上调绒毛长度/隐窝深度比,改善环磷酰胺所致小鼠肠道机械屏障受损状况;提高IFN-γ/IL-4,改善Th1/Th2平衡。仿刺参与梅花参可以显著促进肠道SIgA的分泌,与其促进小肠IgA表达增多有关。结论:4种常见海参均可改善环磷酰胺诱导的小鼠肠黏膜损伤,且以仿刺参及梅花参效果较显著。Abstract： Objective： To investigate the protective effect and mechanisms of four common species of sea cucumbers on cyclophosphamide （CTX）-induced intestinal mucosa damage. Methods： Seventy mice were randomly divided into seven groups, including normal group, model group, positive control group, Apostichopus japonicus group, Isostichopus badionotus group, Thelenota ananas group and Acaudina molpadioidea group. A mouse model of intestinal mucosal damage was induced by CTX injection. The changes in body weight, spleen index, thymus index, villus （V） length, crypt （C） depth and V/C ratio were detected. SIgA, IFN-7 and IL-4 contents in intestinal mucosa were detected by ELISA and IFN-y/IL-4 ratiowas calculated. IgA protein expression was detected by Western blot. Results： Compared with the model group, spleen index, V/C and IFN-y/IL-4 ratio in mice administered with sea cucumbers were significantly improved. The secretion of SIgA were dramatically enhanced in the mice from Apostichopus japonicus and Thelenota ananas groups when compared with the mice from the model group, which was correlated with the enhancement of IgA expression. Conclusion： All four common species of sea cucumbers can significantly protect the intestine mucosa in mice from CTX-induced damage, and Apostichopus japonicas and Thelenota ananas are more effective.

关 键 词：海参 环磷酰胺 肠道黏膜免疫 TH1 TH2 V C值 SIGA 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]