响应面法优化去内毒素乳铁蛋白纯化工艺的研究  

Optimization of purification conditions of endotoxin-free lactoferrin by response surface methodology

作　　者：王浩[1] 刘会平[1] 董丹[1] 张涛[1] 杨洁芳[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中国乳品工业》2013年第7期16-19,共4页China Dairy Industry

基　　金：天津市科技计划项目资助(082H N 2N C 02500)

摘　　要：通过采用离子交换色谱法来去除乳铁蛋白中的内毒素,通过鲎试剂来检测内毒素的含量,并使用响应面法优化了乳铁蛋白的纯化工艺。结果表明:去内毒素后乳铁蛋白中内毒素从原来的18.26 EU/mL降低为0.77 EU/mL,在洗脱剂pH值为4.83,洗脱速度1.21 bV/h,洗脱剂浓度0.05 mol/L醋酸钠缓冲溶液条件下,蛋白得率可达到最大值62.87%。The study showed a method of removing endotoxin from lactoferrin by ion exchange chromatography. Endotoxin was detected by TAL （Tachypleus Amebocyte Lysate）. And the purification conditions was optimized by repose surface methodology. The result showed that the endotoxin content of endotoxin-free lactoferrin was reduced from 18.26 EU/mL to 0.77 EU/mL. Under the condition of eluent pH 4.83, velocity of flow 1.21 bV/h and the eluent concentration 0.05 mol/L NaAc, the optimal value of protein yield was 62.87%.

关 键 词：乳铁蛋白 去内毒素 离子交换 响应面法 

分 类 号：Q936[生物学—微生物学]