2011—2012年广东省市售牡蛎中诺如病毒污染调查分析  

Survey on Norovirus contamination in commercial oysters in Guangdong province from 2011 to 2012

作　　者：梁辉[1] 蒋琦 戴光伟[1] 莫艳玲[1] 方苓[1] 李晖[1] 闻剑[1] 林协勤[1] 胡志坤[1] 邓小玲[1] 黄琼[1] 张永慧[1] 

机构地区：[1]广东省疾病预防控制中心,广东广州511430 [2]广东省公共卫生研究院,广东广州511430

出　　处：《中国食品卫生杂志》2013年第4期359-362,共4页Chinese Journal of Food Hygiene

基　　金：广东省食品安全卫生应急技术研究中心基金(粤科函社字[2011]733号)

摘　　要：目的调查2011—2012年广东省市售牡蛎中诺如病毒的污染状况,为采取有效措施减轻牡蛎中诺如病毒污染程度提供建议,达到预防和控制食用牡蛎引起诺如病毒胃肠炎疾病的目的。方法 2011年3月—2012年10月,在广东省部分沿海城市进行市售牡蛎的采样检测,对其诺如病毒污染状况进行连续两年监测,采用荧光RT-PCR检测诺如病毒阳性标本基因分型,并对不同城市、季节及场所牡蛎中诺如病毒的污染情况及基因分型情况进行比较。结果牡蛎中诺如病毒总检出率为14.9%(41/275);四个季节牡蛎中诺如病毒检出率依次为4.4%、15.7%、18.2%和36.7%;从基因分型分析,GⅠ型病毒株检出率为4.0%,GⅡ型病毒株检出率为6.2%,GⅠ和GⅡ型病毒株同时检出率为4.7%。结论 2011—2012年广东省市售牡蛎的诺如病毒污染情况在市场、餐饮场所以及不同地区间比较差异无统计学意义,其基因分型间比较差异无统计学意义,但呈现明显季节性特点,以冬季污染最严重。Objective To investigate the presence and contamination levels of Norovirus（NoV） in commercial oysters in Guangdong Province from 2011 to 2012,provide suggestions on reducing NoV contamination in oysters,and to prevent and control gastroenteritis outbreaks caused by NoV.Methods Between March 2011 and October 2012,oyster samples collected from several coastal cities in Guangdong Province were examined for NoV by real-time RT-PCR.The contamination levels and genotype of different cities,seasons and sampling sites were compared.Results The overall detection rate of NoV was 14.9%（41/275）.In addition,the detection rate was 4.4%,15.7%,18.2% and 36.7% in spring,summer,autumn,and winter,respectively.Among 275 samples,4.0% belonged to GI,6.2% GII,and 4.7% both GI and GII.Conclusion No significant differences were found in NoV contamination levels and genotye among markets,restaurants,and various cities from 2011 to 2012.However,there were obvious differences in different seasons,especially in winter whick had the highest NoV contamination level in oyster.

关 键 词：广东省市售牡蛎 诺如病毒 污染调查 基因分型 食源性病原体 

分 类 号：R155.5[医药卫生—营养与食品卫生学]