一株拮抗酵母的筛选鉴定及其对草莓采后病害的生防效果(英文)  

Screening and Identification of an Antagonistic Yeast and Its Application for Biological Control of Postharvest Decay of Strawberries

作　　者：靳莎莎[1] 庞水秀[1] 赵利娜[1] 张红印[1] 张晓云[1] 束兆林[2] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏丘陵地区镇江农业科学研究所,江苏镇江212013

出　　处：《食品科学》2013年第13期202-208,共7页Food Science

基　　金：江苏省科技支撑计划项目(BE2011395);江苏省物理加工重点实验室开放基金项目(JAPP2011-1);江苏高校优势学科建设工程资助项目

摘　　要：从果园土壤中分离得到一株具有潜在生防效果的拮抗菌,经分子生物学鉴定及生物化学实验检验,确定其为卡利比克毕赤酵母。该拮抗菌对草莓采后灰霉病和根霉病具有显著的抑制效果,使用拮抗菌的浓度对生物防治效果有显著影响:拮抗菌浓度越高,腐烂率越低。当卡利比克毕赤酵母浓度为1×109cells/mL,灰葡萄孢和匍枝根霉孢子浓度分别为1×105spores/mL和5×104spores/mL时,20℃相对湿度92.5%培养3d,灰霉病和根霉病的腐烂率被完全抑制。该株酵母20℃第1天,4℃前5d能够在伤口处快速增殖。20℃保存5d卡利比克毕赤酵母能够显著腐烂的发生且对草莓本身的品质参数并没有影响。The aim of this research was to isolate a strain of potential antagonistic yeast from soil samples collected from unsprayed orchards,and to test the control efficacy against postharvest decay of strawberries.By molecular biological identification and biochemical examination,the antagonistic strain was identified as Pichia caribbica.The antagonist showed significant biological control efficacy against postharvest decay of strawberries caused by gray mold and Rhizopus which was significantly affected by its concentration： the higher the concentration of the antagonist,the lower the disease incidence.P.caribbica at 1× 10 9 cells /mL was able to inhibit completely gray mold decay and Rhizopus decay of strawberries after 3 d incubation at 20 ℃,while the control fruit showed 92.5% decay incidence when challenged with B.cinerea spores suspension of 1 × 10 5 spores/mL,and 100% decay incidence when challenged with R.stolonifer spores suspension of 5 × 10 4 spores/mL,respectively.Rapid growth of the antagonist in wounds was observed during the first day at 20 ℃,and during the first 5 d at 4 ℃.P.caribbica significantly reduced the natural development of decay and did not impair the fruit quality when stored at 20 ℃ for 5 d.

关 键 词：筛选 鉴定 卡利比克毕赤酵母 采后腐烂 草莓 生物防治 

分 类 号：Q939.9[生物学—微生物学]