刺梨三萜对人肝癌SMMC-7721细胞增殖的影响  

Effect of Rosa roxburghii Tratt Triterpene on Proliferation of Human Hepatoma SMMC-7721 Cells

作　　者：黄姣娥[1] 江晋渝[2] 罗勇[2] 戴支凯[1] 

机构地区：[1]桂林医学院药学院药理学教研室,广西桂林541004 [2]贵州航天医院神经内科,贵州遵义563000

出　　处：《食品科学》2013年第13期275-279,共5页Food Science

基　　金：广西高校优秀人才资助计划项目(桂教人[2009]62号)

摘　　要：目的:观察刺梨三萜(Rosa roxburghii Tratt triterpene,RRTT)对人肝癌细胞SMMC-7721细胞增殖的影响,研究其可能的作用机制。方法:采用形态学观察和MTT法分析RRTT对SMMC-7721细胞增殖的影响;通过NBT还原实验和细胞培养上清液中甲胎蛋白(alpha-fetoprotein,AFP)含量的测定分析RRTT对SMMC-7721分化的影响;采用AO/EB染色法和FCM检测RRTT对肿瘤细胞周期和细胞凋亡的影响;RT-PCR检测Bad mRNA的表达。结果:RRTT对SMMC-7721细胞增殖的抑制作用呈时间和剂量依赖性。与阴性对照组比,随RRTT剂量的增加,NBT阳性细胞比率增加,AFP含量逐渐降低。RRTT对SMMC-7721细胞凋亡和增殖周期均无明显影响。RRTT作用后,Bad mRNA的表达下调。结论:RRTT具有体外抗SMMC-7721作用,其机制可能通过下调Bad mRNA的表达而诱导细胞分化,而与抑制细胞增殖和诱导细胞凋亡无关。Objective： To explore the proliferative effect and potential mechanisms of Rosa roxburghii Tratt triterpene（RRTT） on human hepatoma SMMC-7721 cells.Methods： Morphological changes,growth curves and MTT assay were used to evaluate the anti-proliferative effect of RRTT on SMMC-7721 cells.Detection of alpha-fetoprotein（AFP） level in cultured medium supernatant and NBT reduction assay were carried out to evaluate the inducive effect of RRTT on the differentiation of SMMC-7721 cells.Cell apoptosis and cell cycle were examined by AO/EB fluorescent staining and flow cytometry.SMMC-7721 cells treated with RRTT were collected for Bad transcript analysis by real-time reverse transcriptase–polymerase chain reaction（RT-PCR）.Results： The inhibitory effect of RRTT on the growth of SMMC-7721 cells tended to be in RRTT dose-dependent and time-dependent manners.The ratio of NBT positive cells revealed an obvious increase and AFP concentration of cultured medium exhibited a decline with increasing RTT dose when compared with the control.No obvious difference in apoptosis or cell cycle distribution of SMMC-7721 cells was observed between the RRTT group and control group.Relative mRNA level of Bad in SMMC-7721 cells was down-regulated by RRTT.Conclusion： RRTT has anticancer effect on SMMC-7721 cells in vitro.Its possible mechanism is due to the cytoxicity and induction on cell differentiation via Bad mRNA down-regulation,rather than the apoptosis and inhibition of cell proliferative cycle.

关 键 词：刺梨 三萜化合物 人肝癌SMMC-7721细胞 细胞增殖 细胞分化 细胞凋亡 

分 类 号：R730.5[医药卫生—肿瘤]